Project description:The goals of this study are to compare different gene expressions for starmerella bombicola Bro1,gme3597 and gme3787 knockout strain, the reference strain wild-type was considered as a control. The deletion of Bro1 significantly downregulated genes involved in the sophorolipids synthesis-related pathwaw and resulted in the inability of the mutant to synthesize sophorolipids. The deletion of gme3696 also down-regulated the expression of sophorolipid synthesis-related genes. The knockout of gme3787 up-regulated the expression of UDP-glucose pyrophosphorylase.This study provides the information that Bro1 function are required in sophorolipids synthesis of starmerella bombicola.
Project description:The presence of tagatose in Lactobacillus rhamnosus strain GG caused induction of a large number of genes associated with carbohydrate metabolism including the phosphotransferase system. In addition, these results indicate the tagatose enhanced the growth of Lactobacillus casei 01 and Lactobacillus rhamnosus strain GG and their probiotic activities by activating tagatose-associated PTS networks.
Project description:The response to bile of Lactobacillus casei BL23 and its derivative strain TC01 was investigated. TC01 strain carries a complete deletion of gene LCABL_02080 which encodes a two component signal transduction response regulator.
