Project description:The goals of this study are to compare different gene expressions for starmerella bombicola Bro1,gme3597 and gme3787 knockout strain, the reference strain wild-type was considered as a control. The deletion of Bro1 significantly downregulated genes involved in the sophorolipids synthesis-related pathwaw and resulted in the inability of the mutant to synthesize sophorolipids. The deletion of gme3696 also down-regulated the expression of sophorolipid synthesis-related genes. The knockout of gme3787 up-regulated the expression of UDP-glucose pyrophosphorylase.This study provides the information that Bro1 function are required in sophorolipids synthesis of starmerella bombicola.
Project description:S. bombicola was fermented under two different nitrogen sources (yeast extract or ammonium sulfate). The fermentation broth after 7 days’ cultivation was taken for extracellular proteins analysis.
