Project description:To determine the role that GrgA plays in chlamydial physiology, we constructed a Chlamydia trachomatis mutant that we term L/cgad-peig, in which the chromosomal grgA (ctl0766 or ct504) has been disrupted by Targetron mutagenesis, and the plasmid carries an inducible grgA under the control of anhydrotetracycline (ATC). RNA-Seq analysis was performed for L2/cgad-peig grown with and without ATC.

2023-11-29 | GSE234589 | GEO

Chlamydia trachomatis 434/Bu

Project description:Chlamydia trachomatis 434/Bu Transcriptome or Gene expression

| PRJNA396761 | ENA

Chlamydia trachomatis L2/25667R

Project description:Chlamydia trachomatis L2/25667R genome sequencing

| PRJEA71105 | ENA

Transcriptional response of human endocervical epithelial A2EN cells to infection with wild-type or CpoS-deficient Chlamydia trachomatis L2/434/Bu

Project description:In a forward genetic screen we identified a Chlamydia trachomatis L2/434/Bu mutant that causes rapid apoptotic and necrotic death of infected host cells. This phenotype could be linked to the loss of the inclusion membrane protein CpoS. We observed a reduction in cell death when infections were carried out in the presence of cycloheximide or actinomycin D, suggesting that the engagement of cell death depends on host transcription and protein synthesis. We applied RNA-Seq to study the transcriptional response of human endocervical epithelial (A2EN) cells to infection with wild-type or CpoS-deficient bacteria. At 6 hpi the transcriptional profiles of cells infected with the two strains were similar to each other and to the transcriptome of uninfected cells. However, by 18 hpi the transcriptional profile of cells infected with the mutant was strongly altered, with 400 host genes being upregulated greater than two-fold. Among those differentially expressed genes, we observed an enrichment of immunity-related genes, including in particular cytokine genes and interferon-stimulated genes. Overall these data demonstrate that the CpoS-deficient mutant induces an exacerbated interferon response.

2017-02-24 | GSE87110 | GEO

Chlamydia trachomatis RC-L2/55

Project description:Chlamydia trachomatis RC-L2/55 genome sequencing project.

| PRJNA66013 | ENA

Quantitative proteomics of the infectious and replicative forms of Chlamydia trachomatis L2

Project description:The obligate intracellular developmental cycle of Chlamydia trachomatis presents significant challenges in defining its proteome. In this study we have applied quantitative proteomics to both the intracellular reticulate body (RB) and the extracellular elementary body (EB) from C. trachomatis. We used C. trachomatis L2 which is a model chlamydial isolate for such a study since it has a high infectivity: particle ratio and there is an excellent quality genome sequence. EBs and RBs (>99% pure) were quantified by chromosomal and plasmid copy number using PCR to determine the concentrations of chlamydial proteins per bacterial cell. RBs harvested at 15h post infection (PI) were purified by three successive rounds of gradient centrifugation. This is the earliest possible time to obtain purified RBs, free from host cell components in quantity, within the constraints of the technology, EBs were purified at 48h PI. We then used two-dimensional reverse phase UPLC to fractionate RB or EB peptides before mass spectroscopic analysis, providing absolute amount estimates of chlamydial proteins.

2016-06-28 | PXD003025 | Pride

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