Project description:Brassica nigra plants, a Brassicaceae close to Arabidopsis thaliana, was used for combined stresses experiments. In this study, we performed a whole-genome microarray analysis on five-week-old plants and compared untreated plants and plants treated different single or dual stresses: the larvae Pieris brassicae, egg extract of Pieris brassicae, the bacterial Xanthomonas campestris pv. raphani, the aphid Brevicoryne brassicae or by combined stresses eggs of P. brassicae / P. brassicae, X. campestris / P. brassicae, B. brassicae / P. brassicae.
Project description:Brassica nigra plants, a Brassicaceae close to Arabidopsis thaliana, was used for combined stresses experiments. In this study, we performed a whole-genome microarray analysis on five-week-old plants and compared untreated plants and plants treated with ozone at 70 ppb, larvae of Pieris brassicae or both ozone followed by P. brassicae insect.
Project description:Plant defence against insects is well known to be affected by previous exposure to cues warning of herbivory. Using Arabidopsis thaliana and the herbivore Pieris brassicae, we addressed the question whether the maintenance of the effects of the warning cue depends on its reliability. We determined the transcriptomes of Arabidopsis leaves that were treated by P. brassicae egg deposition (i) five days after oviposition, (ii) one day after removal of the eggs following the egg treatment, (iii) three days after removal of the eggs, (iv) after two days of herbivory that started one day after removal of the eggs; or that were treated by chilling (v) five days after transfer to 4°C, (vi) one day after transfering the plants to 20°C following the chilling treatment, (vii) three days after transfering the plants to 20°C, (viii) after two days of herbivory that started one day after transfering the plants to 20°C. Arabidopsis thaliana Col-0 wild type plants were grown under short day conditions (10 h/14 h light/dark) at 20°C for 7 weeks. Subsequently (i) the plants were transferred to 4°C for 5 days, or (ii) Pieris brassicae deposited ca. 40 eggs on leaf 17 where they remained for five days, or (iii) as controls plants grew untreated for five days. Next, the plants were transferred back to 20°C and the eggs were removed, respectively. Next, all plants rested for 1 day at 20°C. Next, P. brassicae larvae were allowed to feed for 2 days on leaf 17 adjacent to the former egg deposit site or at a respective leaf region of chilling-treated or untreated plants. Control plants were not exposed to larvae. From all treated and untreated plants material from a leaf region proximal to the egg deposition and/or feeding site was harvested for transcriptome analysis.
Project description:Arabipdosis thaliana (ecotype Col-0) was infected with the root pathogen Plasmodiophora brassicae. Gene expression of the host plant has been analyzed at two time points after inoculation (10 and 23 days after inoculation) compared to the correspondend control plants.
Project description:Plants within the Brassicaceae family have a unique defence mechanism known as the “glucosinolate-myrosinase” system. Upon tissue disruption by insect herbivores, glucosinolates are hydrolysed by the enzyme myrosinase (EC 18.104.22.168) into a variety of degradation products, which can deter insect herbivory. This process has been termed as “The Mustard Oil Bomb”. Seeds of Brassica napus have been genetically modified to remove myrosinase containing myrosin cells. The modified plants have been named MINELESS due to a lack of toxic mines in seeds. This study aimed to get insights into defence responses of B. napus wild-type and MINELESS seedlings, after being challenged by larvae of the generalist herbivore Mamestra brassicae. The microarray analysis showed 494 and 159 genes to be differentially regulated after M. brassicae feeding on wild-type and MINELESS seedlings, respectively. Many of the observed transcriptional responses i B. napus and the MINELESS mutant are related to those found in Arabidopsis thaliana plants when they are exposed to insects. Overall design: Mamestra brassicae larvae were maintained and propagated on wild-type (Brassica napus) and MINELESS (Brassica napus) seedling for 10 days. The 10-days old M. brassicae larvae were transferred to 6-7 days old seedlings of wild-type and MINELESS, respectively. In order to retain the larvae, pots (4 seedlings in each pot) were enclosed in cages. Controls were treated in the same way, without insects. Pots with control (non-infested) and M. brassicae infested seedlings were kept under the same conditions in greenhouse. Control and M. brassicae challenged seedlings were harvested after 24h of induction. Cages and larvae were removed and seedlings were harvested and flash frozen in liquid nitrogen. Each biological replica of untreated plants (controls) comprised 4 pots (4 seedlings in each pot), while for M. brassicae challenged seedlings, each biological replica comprised 6 pots (4 seedlings in each pot). Differences in transcriptional responses were measured by comparing genes expression of M. brassicae challenged seedlings against non-infested control seedlings.