Project description:The yeast Pichia caribbica shown the capability to degrade patulin by the intracellular enzymes. However, the enzymes which risponsible for the degradation process was unkonown. Transcriptome change in response to mycotoxin patulin was analyzed. The molecular mechanism of Pichia caribbica withstand patulin was revealed.
Project description:Pichia caribbica, which was isolated by our laboratory, was cultured at 4 °C on nutrient yeast dextrose agar (NYDA).A loop of the culture was inoculated in nutrient yeast dextrose broth and incubated on a rotary shaker at 180 rpm at 28 °C for 20 h.
