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Bicoid binding along anteroposterior axis of Drosophila Melanogaster embryos

ABSTRACT: Bicoid binding along anteroposterior axis of Drosophila Melanogaster embryos

PROVIDER: PRJNA404072 | ENA |

REPOSITORIES: ENA

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Bicoid binding along anteroposterior axis of Drosophila Melanogaster embryos

Project description:ChIP-Seq against Bicoid on manually dissected posterior thirds of Drosophila Melanogaster early stage 5 embryos.

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RNAseq of mouse presomitic mesoderm segments along the anteroposterior embryo axis.

Project description:RNAseq of mouse presomitic mesoderm segments along the anteroposterior embryo axis.

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RNAseq of mouse presomitic mesoderm segments along the anteroposterior embryo axis.

Project description:Somitogenesis is the segmentation of the developing embryonic body axis into somites and is guided by oscillating genes, which create waves of expression that travel across the presomitic mesoderm (PSM) from posterior to anterior. Upon arrival of a wave at the PSM's anterior end, a new somite is formed. To identify genes that are expressed in a wave-like pattern we dissected the PSM of four different mouse embryos (pre-turned), separated the left and right sides, and divided each into five segments, from posterior to anterior (sampling sites 1 to 5). Each segment was used to construct libraries for high-throughput RNA-sequencing. For one embryo, we also sequenced two somites.

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Transcriptional differentiation along the longitudinal axis of the developing hippocampus.

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Project description: Not available

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Spatiotemporal transcriptional dynamics of the cycling mouse oviduct

Project description:Female fertility in mammals requires the iterative remodeling of the entire adult female reproductive tract across the menstrual/estrous cycle. Here we examine global transcriptional dynamics of the mouse oviduct along the anteroposterior axis and across the estrous cycle. Though we observed robust patterns of differential oviduct gene expression along the anteroposterior axis, we found surprisingly few changes in gene expression across the estrous cycle, in marked contrast to other mammals. We speculate that this is an evolutionarily derived state that may reflect the extremely rapid five-day mouse estrous cycle.

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The brain regulatory program predates central nervous system evolution

Project description:Understanding how brains evolved is critical to determine the origin(s) of centralized nervous systems. Brains are patterned along their anteroposterior axis by stripes of gene expression that appear to be conserved, suggesting brains are homologous. However, the striped expression is also part of the deeply conserved anteroposterior axial program. An emerging hypothesis is that similarities in brain patterning are convergent, arising through the repeated co-option of axial programs. To resolve whether shared brain neurogenic programs likely reflect convergence or homology, we investigated the evolution of axial programs in neurogenesis. We show that the bilaterian anteroposterior program patterns the nerve net of the cnidarian Nematostella along the oral-aboral axis arguing that anteroposterior programs regionalized developing nervous systems in the cnidarian-bilaterian common ancestor prior to the emergence of brains. This finding rejects shared patterning as sufficient evidence to support brain homology and provides functional support for the plausibility that axial programs could be co-opted if nervous systems centralized in multiple lineages

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Drosophila esc mutants show Hox-derepression along the AP axis in CNS

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RNA-Seq analysis of localization along animal-vegetal axis of Xenopus laevis

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