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Synechococcus elongatus PCC 7942 = FACHB-805

ABSTRACT: Analysis of gene expression in wild type and the rel- strain of Synechococcus elongatus PCC7942 - RNA sequencing during exposure to darkness (cells grown in light/dark conditions)

PROVIDER: PRJNA404081 | ENA |

REPOSITORIES: ENA

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Analysis of gene expression in wild type and the rel- strain of Synechococcus elongatus PCC7942 - RNA sequencing during exposure to darkness (cells grown in light/dark conditions)

Project description:We investigated the role of ppGpp in Synechococcus elongatus PCC7942 in light/dark conditions. We performed RNA-seq experiments using wild type and the rel- strain at dusk and during exposure to 12 h of darkness. We show that ppGpp is required for correct response to darkness.

2017-12-12 | GSE103704 | GEO

Synechococcus elongatus PCC 7942 = FACHB-805

Project description:Analysis of gene expression in the rel- + relA+ and rel- + relAE335Q strains of Synechococcus elongatus PCC7942 with RNA sequencing during constant light conditions

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Analysis of RNA polymerase stalling index in wild type and the rel- strain of Synechococcus elongatus PCC7942 - chromatin immunoprecipitation followed by sequencing during exposure to darkness (cells grown in light/dark conditions)

Project description:We investigated the role of ppGpp in Synechococcus elongatus PCC7942 in light/dark conditions. We performed ChIP-seq experiments using wild type and the rel- strain at dusk, during exposure to 12 h of darkness and at dawn. We show that the RNA polymerase pausing index is globally lower in the rel- strain.

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Synechococcus elongatus PCC 7942 = FACHB-805

Project description:Analysis of gene expression in the wild type and rel- strains of Synechococcus elongatus PCC7942 with RNA sequencing during constant light conditions

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Analysis of gene expression in the wild type and rel- strains of Synechococcus elongatus PCC7942 with RNA sequencing during constant light conditions

Project description:Rel is an enzyme involved in the metabolism of ppGpp. We investigated the role of ppGpp in Synechococcus elongatus PCC7942 in constant light and after exposure to darkness. We performed RNA-seq experiments using the rel- strain and wild type and show that ppGpp is required for regulation of global transcription in light and in darkness.

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Analysis of gene expression in the rel- relA+ and the rel- relAE335Q strain of Synechococcus elongatus PCC7942 - RNA sequencing during exposure to darkness (cells grown in light/dark conditions)

Project description:We investigated the role of basal ppGpp levels in Synechococcus elongatus PCC7942 in adaptation to darkness. We performed RNA-seq experiments using the rel- relA+ and the rel- relAE335Q strain at dusk and during exposure to 12 h of darkness. We show that basal ppGpp levels is not sufficient for restoration of appropriate transcriptional shutdown in response to darkness.

2017-12-12 | GSE105774 | GEO

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Project description:Analysis of gene expression in the "clock rescue" and rpaA- "clock rescue" strains of Synechococcus elongatus PCC7942 with RNA sequencing in light/dark conditions

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Synechococcus elongatus PCC 7942 = FACHB-805

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Project description:Gene expression in the wild type + relA+ and wild type + relAE335Q strains of Synechococcus elongatus PCC7942 after induction of expression of RelA+ or RelAE335Q with theophylline at subjective dusk - RNA sequencing during constant light conditions

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