Project description:Uveal melanoma cell lines and short-term cultures of human uveal melanoma cells from biopsy samples were treated with 100 nM FR900359 or vehicle. RNA-seq was performed.
Project description:To disclose the regulatory role of H3K79me2 in gene expression, we performed RNA-seq of cells treated with DOT1L inhibitor or not and RNA-seq of normal and uveal melanoma cells.
Project description:SF3B1 is coding an essential splicing factor. This gene was found recurrently mutated in uveal melanoma. To understand the consequences of these hotspot SF3B1 mutations, we performed high coverage RNA-seq on 74 primary uveal melanomas, which were treated by primary enucleation. We analyzed data for aberrant splicing in relation with SF3B1 status.
Project description:FR900359 is an allosteric inhibitor of Gq. 92.1 uveal melanoma cells, which are driven by constitutively active Gq, were treated with 100 nM FR900359 or vehicle for 1 day and 3days. RNA-seq was performed.
