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Desulfovibrio vulgaris str. Hildenborough

ABSTRACT: Gene expression of Desulfovibrio vulgaris Hildenborough (DvH) strain ES10-5 evolved under salt stress for 5000 generations

PROVIDER: PRJNA407069 | ENA |

REPOSITORIES: ENA

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Gene expression of Desulfovibrio vulgaris Hildenborough (DvH) strain ES10-5 evolved under salt stress for 5000 generations

Project description:Investigation of gene expression changes in a DvH genotype ES10-5, a strain isolated from population ES10 which has been evolved under salt stress for 5000 generations. The gene expression was compared to a gentype ES9-11 isolated from ES9 evolved under the same condition for 1200 generations and the ancestral strain. The genotype ES10-5 was characterized in this study. ES9-11 was isolated and characterized in Zhou A et al., 2013. Characterization of NaCl tolerance in Desulfovibrio vulgaris Hildenborough through experimental evolution. ISME J, 7(9), 1790-1802

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Cell wide response to air exposure in Desulfovibrio vulgaris Hildenborough

Project description:We investigated the effects of air exposure on Desulfovibrio vulgaris Hildenborough using microarrays. Keywords: stress response, time course

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Cell wide response to low oxygen exposure in Desulfovibrio vulgaris Hildenborough

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Desulfovibrio vulgaris str. Hildenborough

Project description:Salt/NaCl stress in Desulfovibrio vulgaris Hildenborough

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Salt (NaCl and KCl) Stress in Desulfovibrio vulgaris Hildenborough

Project description:Recent interest in the ability of Desulfovibrio vulgaris Hildenborough to reduce, and therefore contain, toxic and radioactive metal waste, has made all factors that affect its physiology of great interest. Increased salinity constitutes an important and frequent fluctuation faced by D. vulgaris in its natural habitat. Using data from microarray experiments, as well as other laboratory analyses, we used a systems approach to explore the effects of excess NaCl on D. vulgaris. This study demonstrates that import of osmoprotectants such as glycine betaine and ectoine constitute the primary mechanism used by D. vulgaris to counter hyper-ionic stress. Several efflux systems also were highly up-regulated, as was the ATP synthesis pathway. Increase in both RNA and DNA helicases suggested that salt stress had affected the stability of nucleic acid base pairing. An immediate response to salt stress included up-regulation of chemotaxis genes though flagellar biosynthesis was down-regulated. Other down-regulated systems included lactate uptake permeases and ABC transport systems. The extensive NaCl stress analysis was compared with microarray data from KCl stress and unlike many other bacteria, D. vulgaris responded similarly to the two stresses. Keywords: Comparison of cells treated with either NaCl (250 mM) or KCl (250 mM) to untreated cells at times of 0, 30, 60, 120, and 240 min.

2006-03-22 | GSE4447 | GEO

Cell wide response to air exposure in Desulfovibrio vulgaris Hildenborough

Project description:We investigated the effects of air exposure on Desulfovibrio vulgaris Hildenborough using microarrays. Keywords: stress response, time course Comparison of cells treated with air to untreated cells at 0,10,30,120,240 min.

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Effect of carbon monoxide and the CO sensor CooA on gene expression in Desulfovibrio vulgaris Hildenborough

Project description:Investigating the role of carbon monoxide and a CO sensor protein CooA in the physiology of Desulfovibrio vulgaris Hildenborough using whole genome expression analysis

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Project description:Salt (NaCl and KCl) Stress in Desulfovibrio vulgaris Hildenborough

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Project description:Different Mutants in Desulfovibrio vulgaris Hildenborough

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