Project description:The carotid body (CB) is a major arterial chemoreceptor containing glomus cells whose activities are regulated by changes in arterial blood contents including oxygen. We employed the single cell RNASeq method by performing next-generation sequencing on single glomus cellderived cDNAs to eliminate contamination of genes derived from other cell types present in the CB. This study has generated a CB expression profile containing novel glomus cell specific genes. The wealth of information provided through this study offers a valuable foundation for identifying molecules functioning in the CB.
Project description:The carotid body (CB) is a major arterial chemoreceptor containing glomus cells whose activities are regulated by changes in arterial blood contents including oxygen. We employed the single cell RNAÂSeq method by performing next-generation sequencing on single glomus cellÂderived cDNAs to eliminate contamination of genes derived from other cell types present in the CB. This study has generated a CB expression profile containing novel glomus cell specific genes. The wealth of information provided through this study offers a valuable foundation for identifying molecules functioning in the CB. 8 single CB glomus cells that were confirmed expressing tyrosine hydroxylase (Th), ubiquitin carboxyl-terminal esterase L1 (Uchl1) and potassium channel subfamily K member 3 (Kcnk3) were harvested from P4-5 wild type mice and subjected to single-cell RNA-Seq
Project description:Transcriptome analysis of Wigglesworthia glossinidia endosymbiont derived from uninfected and infected samples at 3 time points (3, 10 and 20 days). Expression profiling by array - Wigglesworthia glossinidia endosymbiont of Glossina morsitans morsitans
Project description:Transcriptome analysis of Wigglesworthia glossinidia endosymbiont derived from control samples with or without parasite contact at 10 days. Expression profiling by array - Wigglesworthia glossinidia endosymbiont of Glossina morsitans morsitans