Parageobacillus thermoglucosidasius C56-YS93
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA40781 | ENA |
REPOSITORIES: ENA
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Project description:The present work aimed at discovering xylose-inducible and glucose-insensitive promoters from Geobacillus thermoglucosidasius DSM 2542. This strategy enabled the pathway from xylose metabolism to riboflavin production activated by xylose but not glucose, so that glucose was mainly used for cell growth while xylose was used for riboflavin production. By performing whole genome transcriptional analysis of G. thermoglucosidasius DSM 2542 with or without 1% xylose, 71 xylose-activated genes were identified which were controlled by 39 putative promoters. 3 experimentally validated xylose-inducible and glucose-insensitive promoters covering a broad range of transcriptional levels were used to activate the extra pathway from xylose metabolism to riboflavin production. Fermentation results showed the good performance of these promoters for riboflavin production improvement comparing to constitutive promoters. Therefore, our strategy could be applicable to the construction of cell factories that can efficiently use natural carbon sources with glucose and xylose components for the production of high-value chemicals.
2021-02-05 | GSE166135 | GEO
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Project description:Ahmad2017 - Genome-scale metabolic model
(iGT736) of Geobacillus thermoglucosidasius (C56-YS93)
This model is described in the article:
A Genome Scale Model of
Geobacillus thermoglucosidasius (C56-YS93) reveals its
biotechnological potential on rice straw hydrolysate
Ahmad Ahmada, Hassan B. Hartmanb, S.
Krishnakumara, David A. Fellb, Mark G. Poolmanb, Shireesh
Srivastavaa
Journal of Biotechnology
Abstract:
Rice straw is a major crop residue which is burnt in many
countries, creating significant air pollution. Thus,
alternative routes for disposal of rice straw are needed.
Biotechnological treatment of rice straw hydrolysate has
potential to convert this agriculture waste into valuable
biofuel(s) and platform chemicals. Geobacillus
thermoglucosidasius is a thermophile with properties specially
suited for use as a biocatalyst in lignocellulosic
bioprocesses, such as high optimal temperature and tolerance to
high levels of ethanol. However, the capabilities of
Geobacillus thermoglucosidasius to utilize sugars in rice straw
hydrolysate for making bioethanol and other platform chemicals
have not been fully explored. In this work, we have created a
genome scale metabolic model (denoted iGT736) of the organism
containing 736 gene products, 1159 reactions and 1163
metabolites. The model was validated both by purely theoretical
approaches and by comparing the behaviour of the model to
previously published experimental results. The model was then
used to determine the yields of a variety of platform chemicals
from glucose and xylose — two primary sugars in rice
straw hydrolysate. A comparison with results from a model of
Escherichia coli shows that Geobacillus thermoglucosidasius is
capable of producing a wider range of products, and that for
the products also produced by Escherichia coli, the yields are
comparable. We also discuss strategies to utilise arabinose, a
minor component of rice straw hydrolysate, and propose
additional reactions to lead to the synthesis of xylitol, not
currently produced by Geobacillus thermoglucosidasius. Our
results provide additional motivation for the current
exploration of the industrial potential of Geobacillus
thermoglucosidasius and we make our model publicly available to
aid the development of metabolic engineering strategies for
this organism.
This model is hosted on
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MODEL1703060000.
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2017-04-12 | MODEL1703060000 | BioModels