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Gene expression profiling in LoVo-P compared with LoVo-C cells

ABSTRACT: Gene expression profiling in LoVo-P compared with LoVo-C cells

PROVIDER: PRJNA407894 | ENA |

REPOSITORIES: ENA

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Gene expression profiling in LoVo-P compared with LoVo-C cells

Project description:The differential expression of mRNA in LoVo-P cells compared with LoVo-C cells was successfully detected using the Arraystar Human LncRNA/mRNAArray V4.0, that used for the global profiling of 20730 human mRNA and 40173 long non-coding RNA (LncRNA) transcripts.

2017-09-20 | GSE103989 | GEO

microRNA array of LoVo cells and LoVo-Small Extracellular Vesicles

Project description:LoVo cells were cultured in sEV-depleted (160,000xg, 16h) complete medium and the supernatant were collected after 72h. sEVs were purified and centrifuged at 100,000xg for 2.5h using a Beckman SW41Ti rotor to know the miRNA in LoVo cells and LoVo-Small Extracellular Vesicles

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microRNA array of LoVo cells and LoVo-Small Extracellular Vesicles

Project description:microRNA array of LoVo cells and LoVo-Small Extracellular Vesicles

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Expression data from human colorectal cancer cell line LoVo

Project description:PEAK1 overexpression vector and negative control vector were transiently transfected into LoVo cells, respectively, and named LoVo-PEAK1 and LoVo-control groups. LoVo cells of different groups were determined to investigate the different expressions of mRNA using a global gene microarray.

2018-01-10 | GSE108947 | GEO

Expression data from Irinotecan resistant LoVo cells comapred to parental LoVo cells

Project description:Irinotecan, an analogue of camptothecin, is frequently used in combination with various anticancer drugs or as a single agent in treatment of colorectal cancer. But drug resistance of tumor is still a major obstacle to overcome for the success of cancer treatment. In this study, We established chronic irinotecan resistant cell line for new marker to increase the sensitivity to irinotecan and investigated gene expression profiles of the irinotecan-resistant colorectal cancer cell line. To create stable CRC cell line chronically resistant to Irinotecan, LoVo cell was exposed to an initial Irinotecan concentration of 0.1 M-NM-<mol/L in RPMI 1640 supplemented with 10% FBS. When the growth of the cultured cells reaches at 80% confluency, cells were passaged twice at same drug concentration to ensure adaptation and then concentration of Irinotecan was sequentially increased in the same manner to 8 M-NM-<mol/L and then we investigated the gene expressions between parental colorectal cancer cell line, LoVo and Irinotecan resistant LoVo cell lines

2014-07-18 | E-GEOD-59501 | biostudies-arrayexpress

Expression data of LoVo cells treated by 10μM asymmetric dimethylarginine (ADMA) for 96h in serum starvation medium

Project description:ADMA is an endogenous metabolite, which is elevated in cancer patients. Previously, we observed that ADMA treatment attenuated serum starvation-induced apoptosis in LoVo cells. However, the biological functions of ADMA in tumor cells are largely unknown. In current study, we used microarray and untargeted metabolic profiling to investigate the global impacts of ADMA on LoVo cells.

2015-07-16 | GSE70976 | GEO

Gene expression changes with LGR5 knockdown in LoVo colon cancer cells

Project description:The goal of this study was to identify gene expression changes in response to loss of LGR5 in the LoVo colon cancer cell line.

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Expression data from LoVo colon cancer lines +/- constitutive LIN28B expression

2010-12-29 | GSE26334 | GEO

Expression data from LoVo colon cancer lines +/- constitutive LIN28B expression

Project description:We sought to elucidate the molecular mechanisms whereby LIN28B functions by comparing the gene expression profile of cells constitutively expressing LIN28B to empty vector controls. Accordingly, we performed microarray analysis on total RNA isolated from empty vector LoVo and LIN28B-expressing LoVo colon cancer cell lines. Constitutive LIN28B expression was achieved in the LoVo (ATCC #CCL-229) colon cancer cell line via retroviral transduction of MSCV-PIG-LIN28B. Contol = empty vector MSCV-PIG.

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Gene expression changes with LGR5 knockdown in LoVo colon cancer cells

Project description:Gene expression changes with LGR5 knockdown in LoVo colon cancer cells

| PRJNA559893 | ENA