Project description:High-resolution Hi-C in metaphase in wild type cells with and without tension, chl4Δ with tension, and sgo1-3A and sgo1Δ without tension
Project description:Genome binding/occupancy profiling of Sgo1 and the condensin subunit Brn1 by high throughput sequencing. S. pombe is included as the calibration genome.
Project description:ChIP-seq profile of Sgo1 binding sites arrested in metaphase I by deletion of the APC/C activator CDC20. The aim of this experiment is to identify whether Sgo1 binding differs in wild type cells and cells deficient of the meiosis I-specific protein Spo13, which is required for retention of pericentromeric cohesin after metaphase I.
Project description:Genome binding/occupancy profiling of Sgo1 and the condensin subunit Brn1 by high throughput sequencing. S. pombe is included as the calibration genome.
Project description:Calibrated Scc1, Sgo1 and Brn1 ChIP-seq in metaphase with and without tension, in strains where convergent genes at pericentromere borderes are rearranged into a tandem orientation