Project description:In the present study, we investigated the effect of Lactococcus kimchii on healthspan using Caenorhabditis elegans as a model animal. The healthspan of the adult C. elegans fed the mixture of E. coli OP50 and Lactococcus kimchii was significantly longer than that of animals fed sole OP50 (control). Transcriptional profiling comparing Lactococcus kimchii- and control-fed animals suggested that genes related to insulin-like signaling pathway were upregulated by Lactococcus kimchii.
Project description:Transcriptonal profiling of Leuconostoc gasicomitatum LMG18811T (wild type) grown in MRS medium with or without heme. Mutant LMG18811T::pSIP1333A (mutating cydB gene which is essential in the respiratory chain) grown in MRS with or without heme. Comparing mutant and wildtype with and without heme.
Project description:Comparing of transcriptonal profiling of Leuconostoc gasicomitatum LMG18811T grown in semi-defined medium with different carbon sources (20 mM glucose, ribose, inosine).
Project description:Transcriptonal profiling of Leuconostoc gasicomitatum LMG18811T (wild type) grown in MRS medium with or without heme. Mutant LMG18811T::pSIP1333A (mutating cydB gene which is essential in the respiratory chain) grown in MRS with or without heme. Comparing mutant and wildtype with and without heme. Two-condition experiments with wild type and cydB mutant. 1) wild type with heme vs wild type without heme (HVV), 2) wild type with heme vs mutant with heme (HMHV) 3) mutant with heme vs mutant without heme (MHM) 4) wildtype without heme vs mutant without heme (MV).
