Project description:A hybrid array setup containing WT Pyrococcus furiosus genes plus heterologous genes for engineered pathway producing 3HP from maltose and CO2 was used to study the impact of heterlogous pathway on native metabolic systems Four different experiments were run: 1) Pfu WT vs Pfu COM1; 2) Pfu COM1 vs MW56 (strain containing 3 heterologous genes from Metallosphaera sedula for production of 3HP); 3) Pfu COM1 vs MW76 at 72C before and after 3HP production; 4) MW76 at 72C before and after 3HP production at high agitation
Project description:Semiconductor sequencing of alkaline degraded total RNA from Pyrococcus furiosus annotated for ”The 23S ribosomal RNA from Pyrococcus furiosus is circularly permuted” published in Frontiers in Microbiology”
Project description:Small RNA Sequencing from Pyrococcus furiosus Keywords: Small RNA Analysis Analysis of Small RNA from one sample of Pyrococcus furiosus
Project description:Analysis of CRISPR RNAs from both total Pyrococcus furiosus RNA and those that co-purify with the Cmr complex, which has been predicted to be involved in RNA-mediated targeting of foreign RNAs in prokaryotes. Small RNAs (20 - 70 nts) were isolated either from total Pyrococcus furiosus RNA or RNAs isolated from immunopurified Cmr complexes. The RNAs were cloned and sequenced using the Illumina platform. Results provide insight into the anatomy of Cmr2-associated CRISPR RNAs.
Project description:The transcriptional regulator TrmBL1 from the hyperthermophilic euryarchaeon Pyrococcus furiosus functions as repressor as well as activator of genes encoding enzymes mainly involved in sugar uptake, glykolysis and gluconeogenesis. The aim of this study was to explore the genome-wide binding sites of TrmBL1 in Pyrococus furiosus by ChIP-seq in vivo. Two different growth conditions were tested. Pyrococcus furiosus was cultured on pyruvate to induce gluconeogenic growth and on starch to induce glycolytic growth.
Project description:Experimentally mapped transcriptome structure of Pyrococcus furiosus DSM 3638 by hybridizing total RNA (including RNA species <200 nt) to genome-wide high-density tiling arrays (60 mer probes tiled every 16 nt).
