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Comparison between ethylene signaling regulated transcriptome with glucose-TOR or glucose-HXK1 regulated transcriptome

ABSTRACT: Comparison between ethylene signaling regulated transcriptome with glucose-TOR or glucose-HXK1 regulated transcriptome

PROVIDER: PRJNA411817 | ENA |

REPOSITORIES: ENA

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Comparison between ethylene signaling regulated transcriptome with glucose-TOR or glucose-HXK1 regulated transcriptome

Project description:There are two principal mechanisms underlie sugar perception and signal transduction in biological systems. Direct sensing and signaling is triggered by sugar direct binding sensors such as Hexokiase 1 (HXK1) with a broad range of affinity and specificity, and indirect sensing signaling via a variety of energy and metabolite sensor, such as Target of Rapamycin (TOR). To investigate the intertwined interaction between glucose and plant hormones ethylene, the transcriptome regulated by ethylene, glucose-TOR, and glucose-HXK1 were analyzed and compared. Our results unexpectedly revealed a deep antagonistic interaction between glucose-TOR-energy signaling and the plant stress hormone ethylene.

2020-09-08 | GSE104146 | GEO

Identification of glucose-TOR signaling early target genes in Arabidopsis seedling autotrophic transition stage

2013-04-05 | GSE40245 | GEO

Identification of glucose-TOR signaling early target genes in Arabidopsis seedling autotrophic transition stage

Project description:The goal of this experiment was to explore the molecular network of glucose-TOR signaling in Arabidopsis seedling autotrophic transition stage. We used the whole-genome microarrays to detail the global program of gene expression mediated by glucose and TOR. Arabidopsis WT and estradiol inducible RNAi-tor seedlings were germinated in low light condition for 3 days to deplete seed nutrient supply. Fifteen mM glucose was adding back to reactivate the quiescent seedlings and the samples were harvested for RNA isolation. Transcript profiles were analyzed and compared between with and without glucose treatment to identify early glucose-TOR target genes. Each experimental condition was repeated three times and total 12 samples were generated.

2013-04-05 | E-GEOD-40245 | biostudies-arrayexpress

protein in ethylene regulated salt response in quinoa

Project description:The experiments were used to detect proteins in ethylene regulated salt response in quinoa. The quinoa is one of healthy food source for human. Ethylene is a stress hormone, which improves salt tolerance in plants by regulating adaptive changes in gene expression level. Proteomic analysis provides an efficient method to excavate downstream functional genes in a large scale. In order to clarify the ethylene regulated salt response pathway in quinoa, the whole seedlings of 4-week-old quinoa treated with water, salt, salt and ethylene precuorsor ACC, respectively, and analyzed by proteomic analysis in this research.

2021-05-23 | PXD026210 | iProX

tor-Transcriptome analysis of TOR (Target of Rapamycin) mutant.

Project description:ra03-07_tor - tor - Study of 2 TOR mutants (7817 Strong, 7846 weak) - Mutant vs wild type on different nitrogen conditions (10mM=control and 100uM) Keywords: treated vs untreated comparison,wt vs mutant comparison

2007-06-20 | GSE7987 | GEO

Differentially regulated genes in TOR knockdown Arabidopsis lines

2012-12-18 | GSE42968 | GEO

Identification of a TOR-regulated phosphoproteome and the regulation of cellular protein synthesis

Project description:We describe an in depth analysis in fission yeast of changes in the phosphoproteome after inhibition of the TOR protein kinases. A total of 756 phosphosites on 316 proteins showed significant and rapid changes in phosphorylation levels after TOR kinase inhibition when the rate of protein synthesis reduced by 90%. These proteins were involved in a range of cellular processes including 4 that featured strongly: signalling-related processes, translation, cell polarity and actin cytoskeletal organisation, and vesicle-mediated transport and autophagy. We propose that these processes have major roles in TOR regulated cellular growth control. We also show that the S6 protein kinases have no role in immediate TOR regulated cellular protein synthesis, but in contrast there is a role for Tif471, the translation initiation factor homologue of eIF4G. This protein becomes rapidly phosphorylated after TORC1 inhibition in particular, but if that phosphorylation is prevented, as demonstrated by mutations on the specific phosphosites, the inhibition of protein synthesis rate is reduced. We propose that this factor is a downstream target of the TOR pathway, with a role in partially regulating global cellular protein synthesis.

2021-07-09 | PXD023664 | Pride

rnai tor time course - The signalization way TOR/PTEN in Arabidopsis

Project description:gnp_blan06_torpten - rnai tor time course - The impact of the TOR pathway on growth and stress responses. Modification of translational and transcriptional profiles in Tor-inducible RNAi mutants and identification of TOR targets.

2011-01-07 | GSE25701 | GEO

The kinetics of ethylene-regulated changes in the root transcriptome

Project description:Ethylene-dependent gene expression was assayed by treating with 1 uM ACC, an ethylene precursor, or a control treatment to Arabidopsis seedlings by transferring 6 day old Arabidopsis grown on a a nylon mesh to fresh ACC-containing or control media Seedling roots were harvested 0, .5, 1, 2, 4, 8, 12, and 24 hours after treatment and the resultant RNA was used for microarray analysis to determine the kinetic profiles of auxin-responsive gene expression

2016-10-01 | GSE84446 | GEO

Differentially regulated genes in TOR knockdown Arabidopsis lines

Project description:Rapamycin-sensitive transgenic Arabidopsis lines (BP12) expressing yeast FK506 Binding Protein12 (FKBP12) were developed. Inhibition of TOR in BP12 plants by rapamycin resulted in slower overall root, leaf and shoot growth and development leading to poor nutrient uptake and light energy utilization. Genetic and physiological studies together with RNA-Seq and metabolite analysis of TOR-suppressed lines revealed that TOR regulates development and lifespan in Arabidopsis by restructuring cell growth, carbon and nitrogen metabolism, gene expression, ribosomal RNA and protein synthesis. Arabidopsis WT (Col)and BP12-2 (TOR knockdown line) seedlings at 15 DAG were treated with rapamycin for 3 days by transferring from 0.5 MS medium to 0.5 MS+10 ug/ml rapamycin. Triplicate samples of rapamycin treated WT and BP12-2 seedlings were used for RNA-Seq analysis (Illumina Hiseq 2000). Paired-end alignments were obtained through aligning short reads onto the reference Arabidopsis Genome (TAIR9) using Bowtie. More than 80% of the reads mapped onto the genome. Htseq-count was used to count the reads from the Bowtie derived output files. Differential expressed genes were identified using edgeR. The FDR-corrected P value for differential expression was set to be <=0.05.

2012-12-18 | E-GEOD-42968 | biostudies-arrayexpress

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