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Expression data from glioblastoma derived sphere lines, adherent cell lines and their original glioblastoma tumor

ABSTRACT: Expression data from glioblastoma derived sphere lines, adherent cell lines and their original glioblastoma tumor

PROVIDER: PRJNA412249 | ENA |

REPOSITORIES: ENA

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Expression data from glioblastoma derived sphere lines, adherent cell lines and their original glioblastoma tumor

Project description:Glioblastoma (GBM) derived sphere lines and adherent cell lines are an important tool for research in basic and translational neuro-oncology. Documentation of their genetic identity has become a requirement for scientific journals and grant applications to exclude cross-contamination and misidentification that lead to misinterpretation of results. Here, we report expression data for 26 samples including 4 GBM derived sphere lines (4 x 3 replicates), 2 GBM derived sphere lines passaged through intracranial transplantation (2x 1), 2 adherent GBM derived cell lines (2 + 2 x 3 replicates), 4 corresponding glioblastoma tumors and 2 non-tumor brain tissues.

2017-09-27 | GSE104291 | GEO

Chondrosarcoma cells: sphere vs adherent

Project description:Transcriptional profiling of human chondrosarcoma sphere vs adherent cells

2015-04-30 | GSE47823 | GEO

Chondrosarcoma cells: sphere vs adherent

Project description:Transcriptional profiling of human chondrosarcoma sphere vs adherent cells Two different growth conditions, sphere vs adherent

2015-04-30 | E-GEOD-47823 | biostudies-arrayexpress

Human colorectal cancer cell line CRC#21: Sphere-cultured cells vs. Adherent-cultured cells

Project description:Transcriptional profiling of cancer stem cells (sphere-cultured cells) comparing non-cancer stem cells (adherent-cultured cells). Goal was to identity cancer stem cell-specific genes. Two-condition experiment, sphere-cultured cells vs. adherent-cultured cells: 1 sphere-cultured replicate and 1 adherent-culture replicate.

2016-04-01 | E-GEOD-77150 | biostudies-arrayexpress

CaSki cells: adherent cultured cell vs. sphere cells

Project description:Transcriptional profiling of human cervical squamous cell carcinoma cell line CaSki comparing adherent cultured cells with spheres cultured in serum free culture medium. Goal was to identify candidate antigens for immunotherapy targeting cancer stem cells. Two-condition experiment, CaSki vs. CaSki-sphere cells.

2015-03-04 | E-GEOD-65499 | biostudies-arrayexpress

Human colorectal cancer cell line CRC#21: Sphere-cultured cells vs. Adherent-cultured cells

2016-04-01 | GSE77150 | GEO

Adherent and Spheroid Glioblastoma Proteomics

Project description:Dendritic cell (DC)-based immunotherapy against glioblastoma multiforme is a novel treatment hope. Glioblastoma stem-like cells are, however, potentially causing immunoresistance. Glioblastoma cells cultured as gliomaspheres show a stem-like phenotype as opposed to classical adherent culture. They are thus a promising antigen source to specifically target glioblastoma stem-like cells via DC therapy and so overcome immunoresistance. Here we study the importance of gliomasphere-specific. Methodologically, we used 7 gliomaspheres, 3 of them patient-derived, as model system. Gliomasphere-specific protein expression was explored via quantitative proteomics.

2019-04-15 | PXD008244 | Pride

Expression data of 3 prostate cancer stem cell primary lines comparing spheres and parental/adherent culture

2010-01-01 | GSE19713 | GEO

Expression data of 3 prostate cancer stem cell primary lines comparing spheres and parental/adherent culture

Project description:Transcriptional profile of PCSC spheres in SCM-1% KO (stem-like cells) vs adherent cultures in PCSC-Celprogen medium (differentiated-like cells) Two-condition experiment: Sphere vs. Parental/adherent cells. Biological replicates: 2 sphere replicates , 2 adherent replicates. Early passaged tumor cells were expanded in adherent conditions and then plated in sphere forming conditions (SCM-1KO% medium at low density) to isolate sphere growing cells.

2010-01-01 | E-GEOD-19713 | biostudies-arrayexpress

Altered expression of microRNAs in PC-3 sphere cells of prostate cancer compared with PC-3 adherent cells

Project description:Metastatic prostate cancer is a leading cause of cancer-related death in men. Cancer stem cells (CSCs) are involved in tumor progression and metastasis, including in prostate cancer. There is an obvious and urgent need for effective cancer stem cells specific therapies in metastatic prostate cancer. MicroRNAs (miRNAs) are an important class of pervasive genes that are involved in a variety of biological functions, especially in cancer. The goal of this study was to identify miRNAs involved in prostate cancer metastasis and cancer stem cells. Several published reports have demonstrated that non-adherent spheres culture is increasingly used as an effective method to enrich and identify stem cells or putative CSCs.In our previous study, we enriched prostate cancer stem cells from PC-3 sphere cells in serum-free suspension culture and characterized their CSCs properties.Thus, we used spheres as a prostate cancer stem cells model to elucidate its metastatic mechanisms. We examined the miRNA expression profiles of PC-3 sphere cells of prostate cancer compared with PC-3 adherent cells by miRNA microarray.

2013-04-01 | E-GEOD-44069 | biostudies-arrayexpress

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