Project description:Asparagus is a nutritionally dense stem vegetable whose growth and development are correlated with its quality and yield. For height of 25 cm asparagus , 17-20 cm is still viable, in which the cells can continue to elongate. When 40 cm, the growth in the 17-20 cm range basically stops. Therefore, we picked periods with significant growth differences for transcriptome comparison to find the dynamic changes and underlying mechanisms during the elongation and growth process of asparagus stems. The results showed that the differentially expressed genes were enriched in many aspects, including the metabolism of plant hormones and carbohydrates.
Project description:Two independent small RNA (sRNA) libraries from male and female asparagus plants were sequenced, generating 4.13 and 5.88 million final cleaned reads, respectively. A total of 154 conserved miRNA belonging to 26 families, and 40 novel miRNA candidates that seemed to be specific to asparagus were identified, among them, 63 miRNAs exhibited significant differential expression between male and female plants, and 36 target mRNAs representing 44 conserved and fournovel miRNA in asparagus by high-throughput degradome sequencing analysis.
