Project description:Transcriptome comparison of the winter malting barley '88Ab536' with the spring malting variety 'Morex' at two time points of the malting process: 'out of steeping' and '3 days of germination'. Three replicates of each genotype and time point were accomplished. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Maria Munoz-Amatriain. The equivalent experiment is BB76 at PLEXdb.]

2011-11-22 | GSE33842 | GEO

Transcription profiling of malting barley seeds

Project description:Malting is seed germination under strictly controlled environmental conditions. Malting quality is a complex phenotype that combines a large number of interrelated components, each of which shows complex inheritance. Currently, only a few genes involved in determining malting quality have been characterized. This study combined transcript profiling with phenotypic correlations to identify candidate genes for malting quality. We used the Barley1 GeneChipÂ® array to identify differentially expressed genes in four malting stages relative to dry seed in the barley variety Morex, and to identify differentially expressed genes among four barley varieties. Experiment Overall Design: Four malting barley cultivars were micromalted: Morex and Legacy (6-row), Merit and Harrington (2-row). Two to three batches of micromalting (biological replications) were performed. For each micromalting experiment, 20 g samples were collected for Morex at four stages: steeping, 24 h germination (day 1), 93 h germination (day 4), and finished malt after kilning was completed. Ungerminated, dry seed of Morex was used as reference sample. In another experiment, seeds of Legacy, Harrington, and Merit were micromalted and 20 g samples were collected for each variety during day 1 and day 4 germination stages. Expression profiles were compared among the four cultivars separately for day 1 and day 4.

2009-03-15 | E-GEOD-11200 | biostudies-arrayexpress

Transcriptome alterations of an in vitro-selected, moderately-resistant, two-row malting barley in response to 3ADON, 15ADON and NIV chemotypes of Fusarium graminearium

Project description:Fusarium head blight caused by Fusarium graminearum is a devastating disease of malting barley. Mycotoxins associated with contaminated grain can be transferred from malt to beer and pose a health risk to consumers. In western Canada, F. graminearum has undergone an adaptive shift from 15ADON constituency to dominance by virulent 3ADON-producers, likewise NIV-producers have established in regions of southern United States. Lack of adapted resistance sources with adequate malting quality has promoted the use of alternative breeding methodologies such as in vitro selection. We studied the low-deoxynivalenol characteristic of in vitro selected, two-row malting barley variety ‘Norman’ by RNAseq in contrast to its parental line ‘CDC Kendall’, when infected by 15ADON-, 3ADON- and NIV-producing isolates of F. graminearum. The current study documents higher mycotoxin accumulation by 3ADON isolates, thereby representing increased threat to barley production. At 72-96 hours post infection, significant alterations in transcription patterns were observed in both varieties with pronounced upregulation of the phenylpropanoid pathway and detoxification gene categories (UGT, GST, CyP450 and ABC) particularly in 3ADON treatment. Defense response was multi-tiered, where differential expression in ‘Norman’ associated with antimicrobial peptides (thionin 2.1, defensing, non-specific lipid-transfer protein) and stress-related proteins such as late embryogenesis abundant proteins, heat-shock, desiccation-related and a peroxidase (HvPrx5). Several gene targets identified in ‘Norman’ would be useful in application of breeding varieties with reduced deoxynivalenol content.

2021-05-08 | GSE174081 | GEO

Structural and functional characterization of a winter malting barley

2011-11-22 | E-GEOD-33842 | biostudies-arrayexpress

Sorghum brewing SWATH-MS proteomics

Project description:Identification and relative quantification of proteins present during sorghum malting and in a sorghum malt and barley malt mash and boil measured by SWATH-MS.

2022-10-21 | PXD034981 | Pride

Barley husk adhesion correlates with changes in gene expression associated with plant cuticle biosynthesis and caryopsis cuticle composition

Project description:Caryopses of barley grains become firmly adhered to the glumes of the husk during grain development through a cuticular cementing layer on the caryopsis surface. The quality of this attachment varies among cultivars, with poor quality adhesion causing “skinning”, an economically significant grain quality defect for the malting industry. Malting cultivars encompassing a range of husk adhesion qualities were grown under a misting treatment known to induce skinning. Changes in gene expression during adhesion development were examined with a custom barley microarray. The abundance of transcripts involved early in cuticular lipid biosynthesis, including acetyl-CoA carboxylase, and all four members of the fatty acid elongase complex of enzymes was significantly higher early in caryopsis development than later. Members of the subsequent cuticular lipid biosynthetic pathways were also higher early in development including the decarbonylation and reductive pathways, and sterol biosynthesis.

2019-05-02 | E-MTAB-7429 | biostudies-arrayexpress

Proteome phenotypes discriminate growing location and malting traits in field-grown barley

Project description:These uploaded files are related to the paper titles: Proteome phenotypes discriminate growing location and malting traits in field-grown barley This paper was accepted for publication in the Journal of Food and Agriculture Chemistry. Uploaded files including: -SWATH-MS data for three barley lines: 006,007,008 in three growing location Toodyay, Mingenew, Munglinup abbreviated as T, Mi and Mun. each sample has 4 replicates. -The FASTA file of the database that we used -Pooled Biological Quality Control sample used for the creation of ion library. -Malting trait data

2022-08-05 | MSV000090084 | MassIVE