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Derivation of hypoblast stem cells from rat embryonic stem cells. [Affymetrix]

ABSTRACT: Derivation of hypoblast stem cells from rat embryonic stem cells. [Affymetrix]

PROVIDER: PRJNA413537 | ENA |

REPOSITORIES: ENA

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Derivation of hypoblast stem cells from rat embryonic stem cells. [Affymetrix]

Project description:Hypoblast stem cells were derived from rat embryonic stem cells (rESCs) by culturing with LIF, EGF, PDGF, and 2% fetal bovine serum. mRNA expression profiles of the cells during derivation at different time points were determined with Affymetrix microarrays. Global transcriptome of rESC-derived hypoblast stem cells (cHypoSCs) was similar to that of rat embryo-derived hypoblast stem cells and rat multipotent adult progenitor cells. In addition, the transcriptome of these cells were compared with the previously published datasets including transcriptome of mouse embryonic stem cells, extraembryonic endoderm cells, single cells of mouse embryos (E3.25, E3.5, and E4.5). The comparison revealed that cHypoSCs have the transcriptome similar to that of extraembryonic endoderm cells and E3.5 primitive endoderm cells.

2019-01-01 | GSE104697 | GEO

Derivation of hypoblast stem cells from rat embryonic stem cells.

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Project description:We report the mRNA profiles of single cells with high-throughput sequencing (RNA-seq) of hypoblast stem cells derived from rat embryonic stem cells. The single-cell data was combined with the previously published dataset: transcriptomes of mouse embryo cells (inner cell mass or trophectoderm). The clustering revealed that rat embryonic stem cell-derived hypoblast stem cells were similar to the mouse inner cell mass cells.

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Derivation of trophoblast stem cells from naïve human pluripotent stem cells [ATAC-seq]

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