Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

H.pylori infections induces a transcriptional signature in gastric eosinophils, as determined by RNA sequencing

ABSTRACT: H.pylori infections induces a transcriptional signature in gastric eosinophils, as determined by RNA sequencing

PROVIDER: PRJNA414920 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

H.pylori infections induces a transcriptional signature in gastric eosinophils, as determined by RNA sequencing

Project description:Eosinophils are highly abundant throughout the gastrointestinal tract. However, their function in bacterially-induced gastrointestinal inflammatory disorders is poorly understood. In this study, we have examined the function and regulation of gastric eosinophils in the steady state and during infection with Helicobacter pylori. We find that eosinophils are recruited to sites of infection, make direct contact with live bacteria, and activate a signature transcriptional program.

2018-06-29 | GSE105143 | GEO

Expression profiling of lncRNAs/mRNA for H.pylori infected normal gastric epithelial cell line

Project description:In this study, cultured H.pylori was used to infect the normal gastric epithelial cell line GES-1, to construct the H.pylori infection model. lncRNA microarray was applied for detecting the lncRNA in H.pylori infection model.

2018-03-14 | GSE111763 | GEO

Air Liquid Interface Model of Gastric Mucosa and H.pylori Infection

Project description:The Air Liquid Interface Model of Gastric Mucosa allows to provide a fully polarized epithelium accessible to infection experiments with H.pylori. The influence of several factors on immune response has been studied.

2018-02-15 | GSE94032 | GEO

The H.pylori CagA oncoprotein induces replication fork collapse and DNA double strand breaks through Fanconi Anemia pathway downregulation

Project description:Although H.pylori infection is known to induce host genomic double strand breaks, (DSBs), the role of CagA, a central virulence factor with oncogenic properties in DSB generation remains unclear. Here, using transcriptomic and proteomic analyses, we show that CagA downregulates the expression of major DNA repair factors such as FANCD2, BRCA1 and BRCA2 of the Fanconi Anemia (FA) pathway of DNA repair and leads to compromised host replication fork speed, stability and toxic DSB induction. Our results highlight the mechanism of CagA-mediated genome instability at the level of replication forks and can be used to devise strategies that mitigate the genotoxic effects of H.pylori-infection.

2022-03-24 | GSE132356 | GEO

RNA sequencing of GES-1 cells co-cultured with H.pylori with high and low Trx1 expression

Project description:GES-1 gastric epithelial cells were used for co-culturing with H.pylori with high and low Trx1 expression, and RNA sequencing was performed on the co-cultured cells to explore transcriptome differences between the two groups, so as to further explore the pathogenic mechanism of Trx1 in H.pylori.

2022-08-31 | GSE186902 | GEO

Metastasis-entrained eosinophils enhance lymphocyte-mediated anti-tumor immunity

Project description:The recognition of the immune system as a key component of the tumor microenvironment (TME) led to promising therapeutics. Since such therapies benefit only subsets of patients, understanding the activities of immune cells in the TME is required. Eosinophils are an integral part of the TME especially in mucosal tumors. Nonetheless, their role in the TME and the environmental cues that direct their activities are largely unknown, especially in metastasis. We report that breast cancer-driven lung metastasis is characterized by resident and recruited eosinophils. Eosinophil recruitment to the metastatic lung was regulated by G protein coupled receptor signaling but independent of CCR3. Functionally, eosinophils promoted lymphocyte-mediated anti tumor immunity. Transcriptome and proteomic analyses identified the TME rather than intrinsic differences between eosinophil subsets as a key instructing factor directing anti tumorigenic eosinophil activities. Specifically, TNF-a/IFN-g-activated eosinophils facilitated CD4+ and CD8+ T cell infiltration and promoted anti-tumor immunity. Collectively, we identify a mechanism by which the TME entrains eosinophils to adopt anti-tumorigenic properties, which may lead to the development of eosinophil-targeted therapeutics.

2021-07-27 | PXD027596 | Pride

Transcriptional profiling of mouse eosinophils identifies distinct gene signatures following cellular activation

Project description:Purpose: we aimed to characterized the transcriptional profile of mouse eosinophils in response to stimuli that are associated with Type 1 and Type 2 environments Methods: Eosinophils were purified from the peritoneal cavity of Il5Tg mice and stimulated with IL-4 (i.e. type 2 activated eosinophils), IFN-γ, and a combination of lipopolysaccharide (LPS, i.e. type 1 activated eosinophils) in the presence of IFN-γ. Thereafter, RNA was obtained and the transcriptome signature following each stimulation was determined using RNA sequencing. Raw sequencing reads were trimmed and filtered using fastp, then aligned to mouse genome assembly (GRCm38) using STAR 2.7.2a. Normalization and differential expression analysis were performed in R 4.0.3 using the package DESeq2. Finally, gene ontology annotations were obtained from Ensembl and pathway graphs were obtained from KEGG. Results: RNAseq analysis revealed marked differences, which were driven by the different stimulating conditions Conclusion: We demonstrate that eosinophils are polarized to distinct phenotypes following distinct stimuli. These findings contribute to the growing understanding regarding the heterogeneity of eosinophils and their possible roles in different diseases.

2021-12-04 | GSE189213 | GEO

Proteomics of gastric mucosal tissues of H.pylori-infected DDIT4 knockout mice and wild-type mice

Project description:Proteomics of gastric mucosal tissues of H.pylori-infected DDIT4 knockout mice and wild-type mice

2023-12-25 | PXD048124 | iProX

Tissue adaptaion of intestinal eosinophils in mice

Project description:To determine how eosinophils adapt to the intestinal environment, eosinophils were sorted from the bone marrow and small intestine and compared by RNA sequencing. We show here that intestinal eosinophils were specifically adapted to their environment and underwent substantial transcriptomic changes. Intestinal eosinophils upregulated genes relating to the immune response and cell-cell communication, extracellular matrix components and metalloproteases, and the aryl hydrocarbon receptor (AHR), a ligand-activated transcription factor with broad functions in intestinal homeostasis.

2022-02-25 | GSE185070 | GEO

RAW264.7 cells versus H.pylori infected RAW264.7 cells

Project description:RAW264.7 cells versus H.pylori infected RAW264.7 cells

| PRJNA635395 | ENA