Project description:To investigate the underlying mechanisms in the the states of apical periodontitis, we extracted RNA from control alveolar bone and alveolar bone under apical periodontitis.
Project description:Single-cell gene expression of mandibular bone marrow cells and mandibular bone marrow cells under the stimulation of apical periodontitis were determined by scRNAseq.
2023-02-11 | GSE212975 | GEO
Project description:Microbiota in rat apical periodontitis model
Project description:Dental pain from apical periodontitis is an infection induced-orofacial pain condition that presents with diversity in pain phenotypes among patients. While 60% of patients with a full-blown disease present with the hallmark symptom of mechanical allodynia, nearly 40% of patients experience no pain. Furthermore, a sexual dichotomy exists, with females exhibiting lower mechanical thresholds under basal and diseased states. We conducted a transcriptomic assessment of periapical biopsies (peripheral diseased tissue) from patients with persistent apical periodontitis. our study found several unique differentially expressed genes (DEGs) between symptomatic and asymptomatic patients, as well as novel candidate genes between sexes within the same pain group.
Project description:Chronic apical periodontitis, typified by inflammatory granulation tissue formation and alveolar bone destruction, is the immune response around the apical root caused by long-term infection and pathogenic stimulation in the root canal. Through the recruitment and infiltration of immune cells and inflammatory mediators, wound healing begins accompanied by the starting of infection. Hence, a comprehensive understanding of biological processes and disease development from the cellular microenvironment in inflammatory periapical areas has important implications.
Project description:Periodontitis patients often develop bacteremia, but there has been little evidence showing that oral bacteria translocate into other organs. We found that bacterial colony formation occurs in a culture of liver and spleen cells of periodontitis-induced mice, and the bacterial species detected in the liver and spleen were found in the oral cavity as well, but not in fecal samples, indicating systemic dissemination of oral bacteria during the breakdown of the oral barrier.
Project description:We investigated the association between subgingival bacterial profiles and gene expression patterns in gingival tissues of patients with periodontitis.
