Project description:Whole transcriptome characterization of the effects of dehydration and rehydration on Cladonia rangiferina, the grey reindeer lichen.

| PRJNA206556 | ENA

Whole transcriptome characterization of the effects of dehydration and rehydration on Cladonia rangiferina, the grey reindeer lichen.

2013-11-25 | GSE47624 | GEO

Comparative transcriptional analysis of Acinetobacter baumannii strain ATCC 17978 and strain 17978hm

Project description:In this study the transcriptomes of Acinetobacter baumannii strains ATCC 17978 and 17978hm were compared. Strain 17978hm is a hns knockout derivative of strain ATCC 17978. Strain 17978hm displays a hyper-motile phenotype on semi-solid Mueller-Hinton (MH) media (0.25% agar). ATCC 17978 and 17978hm from an 37C overnight culture were transferred to the centre of the semi-solid MH plate and incubated at 37C for 8 hours. Only 17978hm cells displayed a motile phenotype and covered the complete surface of the plate. These motile 17978hm cells and the non-motile wild-type ATCC 17978 cells were harvested and RNA was isolated. The comparative transcriptome analysis was performed using the FairPlay labeling kit and a custom made Agilent MicroArray with probes designed to coding regions of the ATCC 17978 genome. The data was analyzed using Agilent GeneSpring GX9 and the significance analysis of microarray MS Excel add-on.

2013-08-05 | GSE40681 | GEO

Cladonia borealis strain:ANT050790

Project description:Cladonia borealis strain:ANT050790 Genome sequencing and assembly

| PRJNA693578 | ENA

Comparative LC/MS-based profiling of L-arginine producing, canavanine resistant Corynebacterium glutamicum strain ATCC 21831 and type strain ATCC 13032

Project description:Corynebacterium glutamicum strain ATCC 21831 is a producer of L-arginine that was created by random mutagenesis. It is resistant to the arginine structural analogue canavanine. In order to identify potential bottlenecks in the biosynthetic pathway that leads to this industrially important amino acid, relative metabolite abundances of biosynthetic intermediates were determined in comparison to the type strain ATCC 13032. An extract of U13C-labeled biomass was used as internal standard, to correct for different ionization efficiencies. Metabolites were identified using the ALLocator web platform.

2015-10-06 | MTBLS128 | MetaboLights

Whole transcriptome characterization of the effects of dehydration and rehydration on Cladonia rangiferina, the grey reindeer lichen.

Project description:We have utilized the raw sequence data from our earlier investigation of the lichen transcriptome to design a custom DNA microarray for C. rangiferina in order to study the transcripts expressed in lichen thallus during dehydration and rehydration. The aim of this study was to identify the genes most differentially expressed during the rehydration and drying processes and also to get a more integrative view of the molecular players who may play roles in the processes required for lichen desiccation tolerance and the rapid re-establishment of photosynthesis through functional annotation. 8 samples with three biological replicates for each sample, altogether 24 samples. D1h samples have been drying for 1 hour, D3h samples for 3 hours, D6h samples for 6 hours and Dry samples for 24 hours. W15m samples have been wetted for 15 minutes, W30m samples for 30 minutes, W1h samples for 1 hour and Wet samples for 3 hours.

2013-11-25 | E-GEOD-47624 | biostudies-arrayexpress

Cladonia uncialis strain:Normore 8774

Project description:Cladonia uncialis strain:Normore 8774 Genome sequencing and assembly

| PRJNA348097 | ENA