Project description:Chromatin immunoprecipiation of H3K4me3 of isolated B cells from healthy human donors and LCLs and RNA-sequencing of extracted RNAs
Project description:We previously mapped ETV1 using ChIP-Seq in GIST48 cells (GSE22441). Here, we map the enhancer landscape marked by histone H3K4me1 and the promoter landscape marked by histone H3K4me3 in GIST48 cells. Crosslink ChIP-Seq of H3K4me1 and H3K4me3 in GIST48 cells
Project description:Chromatin immunoprecipitation with massively parallel DNA sequencing (ChIP-seq) was then performed to establish the H3K4me3 landscape in neonatal and adult CD14+ monocytes. As development progressed from neonate to adult, monocytes gained the activating mark H3K4me3. The decreased H3K4me3 abundance at immunologically important neonatal monocyte gene promoters correlated with reduced gene expression, providing evidence that neonatal immune cells exist in an epigenetic state that is distinctly different from adults, and that this state contributes to neonatal specific immune responses.
Project description:We previously mapped ETV1 using ChIP-Seq in GIST48 cells (GSE22441). Here, we map the enhancer landscape marked by histone H3K4me1 and the promoter landscape marked by histone H3K4me3 in GIST48 cells.
Project description:To characterize human bone marrow plasma cells that express or lack CD19 on a molecular level, we compared the global gene expression of primary CD38high/CD138+ plasma cells with or without CD19 expression.
Project description:We describe the landscape of H3K4me3 in WT and jhd2Î? cells in YAR media by ChIP seq To determine the contribution of Jhd2 to the H3K4me3 landscape in respiring yeast cells, we performed H3K4me3 ChIP and normalized to pan-H3 ChIP signal.
Project description:Background: The human CD19 antigen is expressed throughout B cell ontogeny with the exception of neoplastic plasma cells and a subset of normal plasma cells. CD19 plays a role in propagating signals from the B cell receptor and other receptors such as CXCR4 in mature B cells. Studies of CD19-deficient patients have confirmed its function during the initial stages of B cell activation, however its role in the later stages of B cell differentiation is unclear. Objective: Using B cells from a newly identified CD19-deficient individual, we investigated the role of CD19 in the generation and function of plasma cells using an in vitro differentiation model.
Project description:In this study gene expression of CD1c+ CD19- blood dendritic cells from healthy subjects were investigated. Keywords: expression profiling by array Blood DCs (CD1c+ CD19-) were isolated from venous heparinized blood of three apparently healthy human volunteers using the CD1c+ (BDCA-1) dendritic cell isolation kit from Miltenyi according to the manufacturer’s instructions. Purity over 90% (95.8±3%) was determined by FACS analysis. RNA was isolated and 1 µg was labeled using the Affymetrix One-Cycle Target Labeling Kit and the biotinylated cRNA used to perform Affymetrix U133 Plus 2.0 expression arrays.