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TRPS1 shapes YAP/TEAD-dependent transcription in breast cancer cells

ABSTRACT: TRPS1 shapes YAP/TEAD-dependent transcription in breast cancer cells

PROVIDER: PRJNA418840 | ENA |

REPOSITORIES: ENA

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TRPS1 shapes YAP/TEAD-dependent transcription in breast cancer cells

Project description:Yes-associated protein (YAP), the downstream transducer of the Hippo pathway is a key regulator of organ size, differentiation and tumourigenesis. Yet, the activity of YAP can also be modulated by Hippo-independent functions. To disclose these Hippo-independent regulators, we performed a genome-wide CRISPR screening approach that identified the transcriptional repressor protein Trichorhinophalangeal Syndrome 1 (TRPS1) as a potent repressor of YAP-dependent transactivation. Using RNA-Sequencing and ChIP-Sequencing approaches we show that TRPS1 globally regulates YAP-dependent transcription by binding to a large set of joint genomic sites, mainly enhancers. Mechanistically, TRPS1 represses YAP-dependent enhancers function by recruiting a spectrum of corepressor complexes to joint sites. Consequently, loss of TRPS1 leads to activation of enhancers due to increased H3K27 acetylation and an altered promoter-enhancer interaction landscape. TRPS1 is commonly amplified in breast cancer which is associated decreased YAP activity and leads to a decreased frequency of intratumoural immune cells. Consistently, depletion of TRPS1 in the syngeneic 4T1 tumour model leads to a strongly decreased tumour growth in vivo. Our study uncovers TRPS1 as a new epigenetic regulator of YAP activity and it connects repression of YAP-dependent enhancer function to breast cancer. For project-related queries, please contact bjoern.voneyss@leibniz-fli.de <mailto:bjoern.voneyss@leibniz-fli.de>.

2018-08-09 | PXD009819 | Pride

TRPS1 shapes YAP/TEAD-dependent transcription in breast cancer cells [4C-seq]

Project description:TRPS1 shapes YAP/TEAD-dependent transcription in breast cancer cells [4C-seq]

| PRJNA418843 | ENA

TRPS1 shapes YAP/TEAD-dependent transcription in breast cancer cells [ATAC-seq]

Project description:TRPS1 shapes YAP/TEAD-dependent transcription in breast cancer cells [ATAC-seq]

| PRJNA418846 | ENA

TRPS1 shapes YAP/TEAD-dependent transcription in breast cancer cells [ChIP-seq]

Project description:TRPS1 shapes YAP/TEAD-dependent transcription in breast cancer cells [ChIP-seq]

| PRJNA418847 | ENA

TRPS1 shapes YAP/TEAD-dependent transcription in breast cancer cells [RNA-seq]

Project description:TRPS1 shapes YAP/TEAD-dependent transcription in breast cancer cells [RNA-seq]

| PRJNA418848 | ENA

TRPS1 shapes YAP/TEAD-dependent transcription in breast cancer cells [ChIP-seq]

Project description:We report the ChIP-Seq profiles for TRPS1 and TEAD1 in MCF7 and T47D cell lines, and for YAP in MCF7 cells.

2018-08-07 | GSE107013 | GEO

TRPS1 shapes YAP/TEAD-dependent transcription in breast cancer cells [4C-seq]

Project description:We report the 4C-Seq profile of the IGFBP3 transcriptional start site in MCF7 and MCF7 TRPS1 CRISPR KO cell lines.

2018-08-07 | GSE107022 | GEO

TRPS1 shapes YAP/TEAD-dependent transcription in breast cancer cells.

Project description: Not available

| S-EPMC6079100 | biostudies-literature

TRPS1 shapes YAP/TEAD-dependent transcription in breast cancer cells [RNA-seq]

Project description:We report the gene expression profiles of TRPS-depleted and YAP 5SA overexpressing breast cancer cell lines.

2018-08-07 | GSE107010 | GEO

TRPS1 shapes YAP/TEAD-dependent transcription in breast cancer cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-08-07 | GSE107023 | GEO

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