Identification of miR-305, a microRNA that promotes ageing, and its target mRNAs in Drosophila
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ABSTRACT:
PROVIDER: PRJNA419195 | ENA |
REPOSITORIES: ENA
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Project description:MicroRNAs (miRNAs) are involved in the regulation of important biological processes. Here, we describe a novel Drosophila miRNAs involved in ageing. We selected eight Drosophila miRNAs, displaying high homology with seed sequences of ageing-related miRNAs characterized in other species, and investigated whether the overexpression of these miRNAs affected ageing in Drosophila adult flies. The lifespan of adults overexpressing miR-305, a miRNA showing high homology with miR-239 in C. elegans, was significantly shorter. Conversely, a reduction in miR-305 expression led to a longer lifespan than that in control flies. miR-305 overexpression accelerated the impairment of locomotor activity, and promoted the age-dependent accumulation of poly-ubiquitinated protein aggregates in the muscle, as flies aged. Thus, we demonstrate that the ectopic expression of miR-305 has a deleterious effect on ageing in Drosophila. To identify the targets of miR-305, we performed RNA-Seq. We discovered several mRNAs encoding antimicrobial peptides and insulin-like peptides, whose expression changed in adults upon miR-305 overexpression. We further confirmed, by qRT-PCR, that miR-305 overexpression significantly decreases the mRNA levels of four antimicrobial peptides. As these mRNAs contain multiple sequences matching the seed sequence of miR-305, we speculate that a reduction in target mRNA levels, caused by ectopic miRNA expression, promotes ageing.
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