Project description:The LIM-homeobox transcription factor islet-1 (ISL1) has been proposed as a marker of cardiovascular progenitor cells. This study investigated whether forced expression of ISL1 in human mesenchymal stem cells (hMSCs) improves myocardial infarction (MI) treatment outcomes. The lentiviral vector EF1α-ISL1 was constructed using the Multisite Gateway System, and used to transduce hMSCs. Overall design: Examination of mRNA in ISL1-MSCs and Ctrl-MSCs
| GSE107923 | GEO
Project description:RNA sequence of ISL1-MSCs and Ctrl-MSCs
Project description:We report the application of next-generation sequencing (NGS) to analysis the gene expression profile among three different cells:SF-MSCs, iPSCs and iPSC-MSCs. Our results shown that iPSC-MSCs were more similar to SF-MSCs than iPSCs. Overall design: Human SF-MSCs were reprogrammed through the non-viral, integration-free episomal approach into induced pluripotent stem cells (iPSCs), which were then subsequently differentiated into MSCs to establish iPSC-MSC lines.
Project description:Analysis of transformed MSCs at gene expression level. The aim in the present study was that overexpression of MYC in the MSCs alter gene expression of MSCs. Results provide important information of the response of overexpresion of MYCs, such as up- or down-regulated specific anabolic/catabolic cellular functions. Total RNA obtained from transformed MSCs or MSC culture cells at different passages.
Project description:Transcriptional profiling of human mesenchymal stem cells comparing normoxic MSCs cells with hypoxic MSCs cells. Hypoxia may inhibit senescence of MSCs during expansion. Goal was to determine the effects of hypoxia on global MSCs gene expression. Two-condition experiment, Normoxic MSCs vs. Hypoxic MSCs.
Project description:Analysis of transformed MSCs at gene expression level. The aim in the present study was that overexpression of MYC in the MSCs alter gene expression of MSCs. Results provide important information of the response of overexpresion of MYCs, such as up- or down-regulated specific anabolic/catabolic cellular functions. Overall design: Total RNA obtained from transformed MSCs or MSC culture cells at different passages.
Project description:Bovine chondrocyte-seeded and mesenchymal stem cell (MSC)-seeded agarose were cultured for 28 days in chemically defined media containing 10 ng/mL TGF-beta3. Chondrogenic differentiated MSCs were compared to chondrocytes at this timepoint and to undifferentiated MSCs harvested at day 0. Donor-matched sets of chondrocytes and MSCs were used, with three donors total.
Project description:Analysis of gene changes in different genes modulation in MSCs and compared to primary human osteosarcoma cells Total RNA isolated from human parental MSCs and from MSCs siRB, OeMyc, SiRb-OeMyc and primary human osteosarcoma cells
Project description:Early osteoinductive bone marrow MSCs (e-MSCs) acquire enhanced hematopoiesis-supportive ability. We performed microarray analysis on e-MSCs. Cell chemotaxis-assosiated genes were positively enriched and cell adhesion-associated genes were negatively enriched compared with control MSCs. The expression of CXCL12 and VCAM1 extremely decreased. Overall design: Microarray analysis was performed on human bone marrow MSCs stimulated with osteogenesis-inducing cocktails (OICS) for 2 days (e2-MSCs) or 5 days (e5-MSCs). Human bone marrow MSCs without the treatment were used for control.
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs. One-condition experment, gene expression of 3A6