Project description:Enhancers are the key information processing units within the cardiac gene regulatory network. We have demonstrated that cardiac enhancers generate non-coding RNAs. RNA sequencing demonstrates that some of these transcripts are polyadenylated, multi-exonic long non-coding RNAs.
2016-11-12 | GSE52310 | GEO
Project description:long non-coding RNA Sequencing of porcine ovarian granulosa cells
Project description:We discovered Daxx can negatively regulated H3K27me3 level via facilitating long non-coding RNA HOTAIR degradation. To further explore the biological consequence and search for affected genes, we used high throughput sequencing technology to demonstrate the enrichment of H3K27me3 on the human genome and target genes repression after depletion of Daxx. This study revealed a novel role of Daxx in regulating histone modifications via modulates long non-coding RNA stability.
Project description:To test the conservation and evolution of long non-coding RNAs across multiple rodent species by transcriptome sequencing and histone modification mapping. Part of experiment series: E-MTAB-867 RNA-Seq, E-MTAB-959 ChIP-Seq.
Project description:Long non-coding RNA HOX transcript antisense RNA (HOTAIR) is involved in human tumorigenesis and dysregulated in hepatocellular carcinoma (HCC). However, the molecular mechanisms underlying HOTAIR functions in HCC are not well understood. Here, an integrated RNA sequencing transcriptomic and quantitative proteomic analysis were employed to systematically explore the regulatory role of HOTAIR in HCC. A total of 673 transcripts and 298 proteins were identified to be dysregulated after HOTAIR inhibition. Bioinformatics studies indicated that the differentially expressed genes (DEGs) and proteins (DEPs) are involved in many biological processes, especially cancer-related signalling pathways.
