Project description:We developed a novel method for RNA-seq in Trypanosomatids called ‘Spliced-Leader Sequencing’ (SL-seq). The method is based on the fact that the 5’ end of Trypanosomatid mRNA is starts with a SL-sequence, and specifically enriches SL-containing RNA prior to sequencing. In this study we compared the performance and functional results obtained with SL-seq to those generated with conventional Illumina Stranded mRNA prep. Therefore we sequenced mRNA of Leishmania donovani logarithmic phase promastigotes, stationary phase promastigotes and intracellular amastigotes with both methods. We also sequenced controlled dilutions of Leishmania RNA with human RNA.
Project description:To further development of our gene expression, we have employed whole genome microarray expression profiling. Human cord blood CD34+ cells from healthy donors was cultured for 2 days with or without SL-13R.
Project description:RNA was isolated from LNCaP-SL after 24 hours of circulating small extracellular vesicles (csEV) single dose treatment (1×10^10 particles/mL).
Project description:Although previous research has established links between strigolactone (SL) and drought, in this study, we used the barley (Hordeum vulgare) SL-insensitive mutant hvd14 (dwarf14) to scrutinize the SL-dependent mechanisms associated with water deficit response. We have employed a comprehensive approach integrating transcriptome, proteome, phytohormone analyses, and physiological data to unravel differences between wild-type and hvd14 plants when responding to drought. Here we deposited control samples, tissue collected at 25DAS (Days after sowing)
Project description:The goal of this experiment was to determine the gene expression changes that occur in the setting of human leukemia cells with acquired resistance to tagraxofusp (SL-401), a recombinant interleukin 3 - diphtheria toxin fusion targeted cytotoxin
Project description:Drought is a harsh abiotic stress, with plants possessing diverse strategies to survive periods of limited water resources. Although previous research has established links between strigolactone (SL) and drought, in this study, we used the barley (Hordeum vulgare) SL-insensitive mutant hvd14 (dwarf14) to scrutinize the SL-dependent mechanisms associated with water deficit response. We have employed a comprehensive approach integrating transcriptome, proteome, phytohormone analyses, and physiological data to unravel differences between wild-type and hvd14 plants when responding to drought.
Project description:To understand the role of SL-signaling components in water stress response, we have carried out comparative expression analysis of the SL-receptor d14-1 mutant and WT plants under dehydration and well-watered (control) conditions. Aligent’s whole Arabidopsis Gene Expression Microarray (G2519F-021169, V4, 4x44K) was used.
Project description:To understand the role of SL-signaling components in water stress response, we have carried out comparative expression analysis of the SL-response max2-3 mutant and WT plants under dehydration and well-watered (control) conditions. Aligent’s whole Arabidopsis Gene Expression Microarray (G2519F-021169, V4, 4x44K) was used.
