Project description:Here, we report the use of Illumina RNA-Seq for investigating the physiology of the digestive-tract microbiome within the medicinal leech, Hirudo verbana. About 12 million cDNA reads were mapped against the genomes of the two dominant members of this simple microbiome. Results suggested that the most abundant, yet uncultured Rikenella-like bacterium forages host mucin glycans and ferments the carbohydrates to acetate that is secreted into the environment. The second dominant symbiont, Aeromonas veronii, appears to utilize the acetate secreted by Rikenella as a carbon and energy source, possibly linking the physiologies of the dominant symbionts. This study demonstrates how RNA-seq can be used to reveal the physiology of a naturally occurring microbiome.
Project description:In this study we used Illumina RNA-seq to identify genes expressed by A. veronii in mid-log phase growth in a rich medium and within the digestive tract of the medicinal leech. Our results shed light on the physiology of A. veronii during colonization of the leech gut.
Project description:In this study we used Illumina RNA-seq to identify genes expressed by A. veronii in mid-log phase growth in a rich medium and within the digestive tract of the medicinal leech. Our results shed light on the physiology of A. veronii during colonization of the leech gut. A comparison of Illumina RNA-seq of A. veronii in vivo versus in vitro.
Project description:The study aims to investigate the relevance of NKp30 receptor in the salivary glands inflammation of Sjogren’s syndrome patients in comparison to sicca patients (control group) analysing the transcriptomic profile of salivary gland tissues.
Project description:<p>Mechanistic genetic and inflammatory endpoints were studied to gain insight into the molecular mechanisms of Sjögren's syndrome. Transcriptomic studies allowed the exploration of molecular alterations that might cause or influence the studied salivary gland pathology and potentially elucidate druggable pathways for intervention.</p>
