Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Analysis of gene expression profile in the control and CHD7-knockdown hiPSC-derived lt-NES cells (scRNA-Seq)

ABSTRACT: Analysis of gene expression profile in the control and CHD7-knockdown hiPSC-derived lt-NES cells (scRNA-Seq)

PROVIDER: PRJNA423024 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Analysis of gene expression profile in the control and CHD7-knockdown hiPSC-derived lt-NES cells (scRNA-Seq)

Project description:CHARGE syndrome is a congenital disorder caused by mutations in Chromodomain Helicase DNA-binding domain 7 (CHD7) gene. We performed single cell RNA-seq analysis in CTRL and CHD7-knockdown lt-NES cells.

2018-03-28 | GSE108275 | GEO

Transcriptome analysis of single cell-derived hiPSC-lt-NES cell clones

Project description:To identify cell-populations within human iPSC-derived long-term self-renewing neural epithelial stem cells (hiPSC-lt-NES cells) which retain capacities to generate undesired grafts, we established single cell-derived hiPSC-lt-NES cell clones and performed gene expression microarray analysis.

2023-06-04 | GSE166051 | GEO

Transcriptome analysis of hiPSC derived lt-NES cells

2023-06-04 | GSE225719 | GEO

Single Cell Transcriptomic Analysis of hiPSC-lt-NES cells

Project description:We performed single-cell RNA-seq of human iPSC-derived long-term self-renewing neural epithelial stem cells (hiPSC-lt-NES cells) using Quartz-seq methods to characterize cellular heterogeneity .

2023-06-04 | GSE166056 | GEO

scRNA-seq of siRNA treated hiPSC-derived islet cells

Project description:scRNA-seq of siRNA treated hiPSC-derived islet cells

2023-09-11 | GSE218547 | GEO

Expression analysis of Chd7 in mouse hematopoietic stem and progenitor cells [microarray]

Project description:Hematopoietic stem and progenitor cell (HSPC) formation and lineage differentiation involve gene expression programs orchestrated by transcription factors and epigenetic regulators. Knockdown of the chromatin remodeler chromodomain-helicase-DNA-binding protein 7 (CHD7) expanded phenotypic HSPCs, erythroid, and myeloid lineages in zebrafish and mouse embryos. CHD7 acts to suppress hematopoietic differentiation in a cell autonomous manner in the embryo and adult. We performed gene expression analysis to determine the expression of chd7 in adult sorted HSPC populations. Loss of Chd7 in long term hematopoietic stem cells (LT-HSCs) results in upregulation of genes that function in hematopoietic system development and function. Genes representative of each blood lineage including erythroid, myeloid, and lymphoid were upregulated, suggesting that Chd7 deficiency results in LT-HSCs that are more primed for multilineage differentiation. Together with the physical and genetic interaction, the data support a model in which CHD7 interacts with and modulates Runx1 activity to provide proper timing and function of HSPCs as they emerge during hematopoietic development or mature in adults. This represents a distinct and evolutionarily conserved control mechanism to ensure accurate hematopoietic lineage differentiation. We used microarrays to detail the expression of Chd7 in adult sorted HSPC populations.

2023-01-01 | GSE84127 | GEO

CROP-seq of hiPSC-derived astrocytes (scRNA-Seq)

Project description:CROP-seq of hiPSC-derived astrocytes (scRNA-Seq)

| PRJNA755725 | ENA

Single-cell RNA sequencing of human liver development and hiPSC models

Project description:scRNA-seq of primary human adult liver, primary human fetal liver (5 post conceptional weeks), EPCAM+ MACS sorted primary human intrahepatic duct cholangiocytes, sequential timepoints of hiPSC-derived hepatocyte-like cells (HLC), sequential timepoints of hiPSC-derived cholangiocyte-like cells (CLC), sequential timepoints of hiPSC-derived hepatic stellate-like cells (HSLC), sequential timepoints of hiPSC-derived endothelial-like cells (ELC), sequential timepoints of hiPSC-derived macrophage-like cells (MLC) and lentiviral transduced hiPSC-derived hepatocyte-like cells.

2022-05-04 | E-MTAB-8210 | biostudies-arrayexpress

Analysis of single cell gene expression profile in human iPSC-derived lt-NES cells

Project description:Analysis of single cell gene expression profile in human iPSC-derived lt-NES cells

| PRJNA562865 | ENA

Robust production of human neural cells by establishing neuroepithelial-like stem cells from peripheral blood mononuclear cell-derived feeder-free iPSCs under xeno-free conditions

Project description:We performed a microarray experiment to analyze the transcriptional profile of human fetal tissue derived neural stem/progenitor cells, human iPSCs, or human iPSC-derived neural stem/progenitor cells generated using xenogenic or xeno-free reagents. Gene expression patterns were compared among human fetal tissue derived neural stem/progenitor cells, human iPSCs, or human iPSC-derived neural stem/progenitor cells generated using xenogenic or xeno-free reagents. Samples with a title including "Xf" (201B7-lt-NES (Xf)-1, 201B7-lt-NES (Xf)-2, 1210B2-lt-NES (Xf)-1,1210B2-lt-NES (Xf)-2, 1231A3-lt-NES (Xf)-1, 1231A3-lt-NES (Xf)-2) correspond to human iPSC-derived neural stem/progenitor cells generated using xeno-free reagents, whereas samples with a title including "Con" (201B7-lt-NES (Con)-1, 201B7-lt-NES (Con)-2) correspond to those generated using xenogenic reagents.

2016-08-22 | E-GEOD-76900 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data