Project description:Oryza sativa VIN3-LIKE2 (OsVIL2) acts as an epigenetic regulator together with Polycomb Repressive Complex2 (PRC2), which mediates trimethylation of histone H3 lysine 27 (H3K27me3) on target chromatin. T-DNA mutants of OsVIL2 displayed abnormal spikelet development. To understand function of OsVIL2 during spikelet development, RNA-sequencing was conducted in developing panicles of wild-type (WT) and osvil2 mutants.
Project description:camta3-1, camta3-2 and WT microarray experiments, statistical and bioinformatics analysis Keywords: expression analysis; mutant versus wild type comparison
Project description:Wild-type and PIK3CA mutant MCF10A cell lines were analyzed using shotgun proteomics to identify proteins that were altered by either 545K or H1047R PIK3CA mutations. Cell lines were digested and fractionated using isoelectric focusing and peptides were analyzed using LC-MS/MS analysis.
Project description:E9.5 yolk sacs were collected from wild type (CD1) and Cdx-mutant (DKO) embryos and processed for RNA-sequencing to identify Cdx-dependent changes in gene expression
Project description:We characterized a rice (Oryza sativa L ssp. indica cultivar 3037) semi-dwarf mutant sd37, in which CYP96B4 gene (Cytochrome P450 96B subfamily) was identified as the target gene by map-based cloning and complementation test. A point mutation in CYP96B4 leads to a substitution of Thr to Lys in the SRS2 region. The sd37 leaves, panicles and seeds are all smaller compared with those of wild-type, and histological analysis showed that the decreased cell number was the main reason for the dwarf phenotype. We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up- and down- regulated genes during this process.