Project description:Cleavage and morphogenesis of Ciona embryos was blocked by treatment with cytochalasin B prior to gastrulation. Drug treated embryos and DMSO treated controls were harvested at stage 19.5 (after control embryos had undergone neurulation and were completing notochord intercalation). Only 55 genes showed a significant difference in expression between the two treatments.
Project description:To assay Tcf functions during early cardiopharyngeal specification, bulk RNAseq were performed to profile the FACS-purified cardiopharyngeal lineage cells isolated from 15 and 18hpf Ciona larvae with CRISPR/Cas9-mediated Tcf mutagenesis.
Project description:The transcriptome of unfertilized eggs of Ciona intestinalis was determined with microarrays. To obtain gene expression profiles of unfertilized eggs, we took 14 biological replicates. For the first through fourth replicates, we also took a technical duplicate. Gene expression profiles of the 8-, 16-, 32-cell embryos were obtained with embryos from the third to fifth animals.
