Project description:This project characterizes the metabolic consequences of the daily physiological rhythms and diel vertical migration for the model subtropical copepod, Pleuromamma xiphias. P. xiphias were collected near the Bermuda Atlantic Time Series in plankton tows at different times of day, representing different parts of their daily vertical migration. Single copepods were isolated from the tows and flash-frozen for proteomics analysis.
Project description:The diel vertically migrating copepod, Pleuromamma xiphias, migrates approximately 600 m daily, up and down in the water column. At the surface, the copepods feed on plankton at night, and then descend to the depths during the day, likely to escape visual predators. Both the migratory behavior and the physiological pathways that are up- and down-regulated throughout the migration are likely under at least partial circadian clock control. This experiment compares the protein abundances of select physiological processes from copepods collected in situ with copepods incubated in the dark to remove the influence of exogenous stimuli on physiology.
Project description:One of the greatest cyclical patterns in the pelagic ecosystem is the daily vertical migration of various zooplankton and fish to depth, a process referred to as diel vertical migration (DVM). DVM is considered to be energetically costly as tiny plankton migrate hundreds of meters in a 24 hour period. To study the metabolic demands of DVM, the copepod Pleuromamma xiphias was collected during upwards and downwards migration off of Bermuda. Data-dependent acquisition on the Q-Exactive detected >1600 proteins, 180 of which were differentially abundant between the two sampling periods.
Project description:Investigating the bacterial communitites associated with Pleuromamma copepods at the Atlantic basin scale using 16S rRNA gene amplicon libraries and microscopy.
