Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Pervasive, coordinated protein level changes driven by transcript isoform switching during meiosis

ABSTRACT: Pervasive, coordinated protein level changes driven by transcript isoform switching during meiosis

OTHER RELATED OMICS DATASETS IN: MSV000081874

PROVIDER: PRJNA428526 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Pervasive, coordinated protein level changes driven by transcript isoform switching during meiosis

Project description:To better understand the gene regulatory mechanisms that program developmental processes, we carried out simultaneous, genome-wide measurements of mRNA, translation and protein through meiotic differentiation in budding yeast. Surprisingly, we observed that the levels of several hundred mRNAs are anti-correlated with their corresponding protein products. We show that rather than arising from canonical forms of gene regulatory control, the regulation of at least 380 such cases—or over 8% of all measured genes—involves temporally regulated switching between production of a canonical, translatable transcript and a 5' extended isoform that is not efficiently translated into protein. By this pervasive mechanism for the modulation of protein levels through a natural developmental program, a single transcription factor can coordinately activate and repress protein synthesis for distinct sets of genes. The distinction is not based on whether an mRNA is induced or not, but rather based on the type of transcript produced.

2018-02-22 | GSE108778 | GEO

Pervasive, Coordinated Protein-Level Changes Driven by Transcript Isoform Switching during Meiosis.

Project description: Not available

| S-EPMC5826577 | biostudies-literature

Pervasive, coordinated protein level changes driven by transcript isoform switching during meiosis in yeast

2018-01-02 | MSV000081874 | MassIVE

Pervasive 3'-UTR isoform switches during mouse oocyte maturation

Project description:Oocyte maturation is the foundation for developing healthy individuals of mammals. Upon germinal vesicle breakdown, oocyte meiosis resumes and the synthesis of new transcripts ceases. To quantitatively profile the transcriptomic dynamics after meiotic resumption throughout the oocyte maturation, we generated transcriptome sequencing data with individual mouse oocytes at three main developmental stages: germinal vesicle (GV), metaphase I (MI), and metaphase II (MII). When clustering the sequenced oocytes, results showed that isoform-level expression analysis outperformed gene-level analysis, indicating isoform expression provided extra information that was useful in distinguishing oocyte stages. Comparing transcriptomes of the oocytes at the GV stage and the MII stage, in addition to identification of differentially expressed genes (DEGs), we detected many differentially expressed transcripts (DETs), some of which came from genes that were not identified as DEGs. When breaking down the isoform-level changes into alternative RNA processing events, we found the main source of isoform composition changes was the alternative usage of polyadenylation sites. With detailed analysis focusing on the alternative usage of 3'-UTR isoforms, we identified, out of 3810 tested genes, 512 (13.7%) exhibiting significant switches of 3'-UTR isoforms during the process of moues oocyte maturation. Altogether, our data and analyses suggest the importance of examining isoform abundance changes during oocyte maturation, and further investigation of the pervasive 3'-UTR isoform switches in the transition may deepen our understanding on the molecular mechanisms underlying mammalian early development.

2021-10-05 | GSE178836 | GEO

Isoform switching events in esophageal adenocarcinoma progression

Project description:Global isoform switching in esophageal adenocarcinoma (EAC) or precursor lesions has not been previously characterized. This form of alternative splicing has recently been recognized as highly prevalent in multiple other cancers. In this study, isoform switch analysis was performed using next generation sequencing of RNA isolated from patient tissues across a histopathological continuum, ranging from low risk Barrett’s esophagus (BE) to BE with advanced dysplasia. Patients were stratified based on esophageal tissue histopathology.

2022-08-17 | GSE193946 | GEO

Meiosis timecourse Rap1

Project description:Meiosis timecourse Rap1

| PRJNA302914 | ENA

Polyadenylation-related isoform switching in human evolution revealed by full-length transcript structure.

Project description: Not available

| S-EPMC8574621 | biostudies-literature

Pervasive 3'-UTR isoform switches during mouse oocyte maturation

Project description:Pervasive 3'-UTR isoform switches during mouse oocyte maturation

| PRJNA741222 | ENA

SUMO is a pervasive regulator of meiosis.

Project description: Not available

| S-EPMC7924959 | biostudies-literature

Rho and NusG suppress pervasive antisense transcription in Escherichia coli

Project description:This SuperSeries is composed of the following subset Series: GSE41936: Rho and NusG suppress pervasive antisense transcription in Escherichia coli [ChIP-chip]. GSE41938: Rho and NusG suppress pervasive antisense transcription in Escherichia coli [tiling array]. GSE41939: Rho and NusG suppress pervasive antisense transcription in Escherichia coli [RNA-seq]. Refer to individual Series

2012-11-01 | E-GEOD-41940 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data