Project description:PEPT-1 is responsible for the uptake of di-and tripeptides form the intestinal lumen into the epithelial cells. Knock-out of pept-1 in C.elegans results in a severe phenotype. Despite a reduction of brood size and a retarded development, pept-1(lg601) depicts increased stress resistance. Therefore, populations of pept1(lg601) as well as N2(Bristol) were synchronized and sampled at various time points during development. Applying 15N metabolic labeling in combination with shotgun LC/MS-MS, we determined the proteome of both strains during development.
Project description:we used short-reads to perform a comparative analysis S. carpocapsae ALL strain IJs and young adults and C.elegans dauer strain N2 and young adult strain him-8.
Project description:In order to examine the misregulation of genes in loss of function alg-1 and alg-2 versus WT, RNA-seq was performed on D5 C.elegans in WT(N2), alg-1(gk214), alg-2(ok304)
