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Expression data from erythroblast (CD71 and GPA positive cells) derived from fetal liver and adult blood

ABSTRACT: Expression data from erythroblast (CD71 and GPA positive cells) derived from fetal liver and adult blood

PROVIDER: PRJNA430073 | ENA |

REPOSITORIES: ENA

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Expression data from erythroblast (CD71 and GPA positive cells) derived from fetal liver and adult blood

Project description:The fetal hemoglobin (HbF) levels were 95.4 ± 1.5% and 4.4 ± 0.2% in fetal liver-derived and adult blood derived-cultured erythrocytes (n=5), respectively. Following RNA isolation from the CD71 high/GPA positive erythroblasts, gene expression analyses were performed using Affymetrix Human Gene 2.0 ST Array. The Affymetrix raw data files of microarray were preprocessed using robust multi-array average method for background correction, log-transformation, and quantile normalization. Differential gene expression between fetal liver derived- and adult peripheral blood derived-erythroblasts was determined using moderated t-statistics implemented in the Limma package. We found the 1366 genes were upregulated by more than 1.5-fold change with adjusted p-value less than 0.05 in fetal liver derived-erythroblasts.

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Immune transcriptome and secretome differ between CD71+ Erythroid Cells from adult bone marrow and fetal liver parenchyma

Project description:In this work, we compared the expression profiles of cytokine genes between fetal liver CECs and adult bone marrow CECs.

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ATAC-seq from erythroblast (ENCSR793RAV)

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Immune transcriptome and cell culture medium cytokines differ between CD71+ Erythroid Cells from adult bone marrow and fetal liver

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Expression data from wildtype and Rb-/- fetal liver at e12.5

Project description:Rb null embryos exhibit defective fetal liver erythropoiesis. We used microarrays to compare Wt and Rb null fetal livers and to analyse gene expression differences which accompany and may underlie Rb null fetal liver degeneration, erythroid failure, and erythropoietic island dissolution. We used microarrays to compare Wt and Rb null fetal livers and analyse gene expression changes which accompany and may underlie fetal liver. Keywords: retinoblastoma, fetal liver, erythroblast, macrophage, cell death

2007-06-05 | GSE6787 | GEO

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