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Distinct patterns of histone acetyltransferase and Mediator deployment at yeast protein-coding genes

ABSTRACT: Distinct patterns of histone acetyltransferase and Mediator deployment at yeast protein-coding genes

PROVIDER: PRJNA430229 | ENA |

REPOSITORIES: ENA

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Distinct patterns of histone acetyltransferase and Mediator deployment at yeast protein-coding genes

Project description:The transcriptional co-activators Mediator and two histone acetyltransferase (HAT) complexes, NuA4 and SAGA, play global roles in transcriptional activation. Here we explore the relative contributions of these factors to RNA polymerase II association at specific genes and gene classes by rapid nuclear depletion of key complex subunits. We show that the NuA4 HAT Esa1 differentially affects certain groups of genes, whereas the SAGA HAT Gcn5 has a weaker but more uniform effect. Relative dependence on Esa1 and Tra1, a shared component of NuA4 and SAGA, distinguishes two large groups of co-regulated growth-promoting genes. In contrast, we show that the activity of Mediator is particularly important at a separate, small set of highly transcribed TATA box-containing genes. Our analysis indicates that at least three distinct combinations of co-activator deployment are used to generate moderate or high transcription levels, and suggests that each may be associated with distinct forms of regulation.

2018-08-15 | GSE109235 | GEO

Distinct patterns of histone acetyltransferase and Mediator deployment at yeast protein-coding genes.

Project description: Not available

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Coordination of Mediator Complex and Histone Acetyltransferase CBP/p300 in Transcriptional Regulation

Project description:Coordination of Mediator Complex and Histone Acetyltransferase CBP/p300 in Transcriptional Regulation

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Coordination of Mediator Complex and Histone Acetyltransferase CBP/p300 in Transcriptional Regulation [RNA-seq]

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ChIP-seq of Mediator subunits in Mediator mutants and wild type yeast

Project description:Mediator subunits Med17 (Srb4) and Med15 (Gal11) were subjected to ChIP in wild type yeast, med18 (srb5), med20 (srb2), and med2 med3 med15 (triple mutant) yeast, all in kin28-anchor away yeast after 1 hr rapamycin treatment to evict Kin28 from the nucleus. This results in inactivation of Kin28, which reduces Mediator turnover at promoters and allows stronger Mediator ChIP signal at promoters. <br></br>Both Med17 and Med15 were tagged with myc and precipitated using the monoclonal antibody against c-myc. Please note that a specific control for rapamycin treatment was not performed in this experiment, as that has been well documented in the literature for this experiment, using what is called the anchor away method. The untagged sample is a control for non-specific immunoprecipitation.

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ChIP-seq of Mediator subunits in Mediator mutants and wild type yeast

Project description:ChIP-seq of Mediator subunits in Mediator mutants and wild type yeast

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Mediator promotes CENP-A incorporation at fission yeast centromeres

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Project description:CBP/p300 is one group of histone acetyltransferases, which catalyze the acetylation of histone lysine in order to loosen the chromatin structure and promote transcription. The multiple-subunits complex MEDIATOR is important for the transcription initiation through participating in the assembly of transcriptional pre-initiation complex (PIC). Our study identified that MEDIATOR interacts with HAC1 and HAC5, and participate in the regulation of transcription coordinately, thereby regulating the development of Arabidopsis. Besides, MEDIATOR, HAC1, and HAC5 may be organized together by the LLPS to regulate transcription.

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