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Next Generation Sequencing Facilitates Quantitative Analysis of Ter cells Transcriptomes

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of Ter cells Transcriptomes

PROVIDER: PRJNA430875 | ENA |

REPOSITORIES: ENA

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Next Generation Sequencing Facilitates Quantitative Analysis of Ter cells Transcriptomes

Project description:Purpose:The purpose of this study is to detect genes Ter-119+CD45−Lin− cells sorted from spleen of tumor-bearing mice and the control cells. Gene expression differences between seven samples could be found using transcriptome profiling (RNA-seq) analysis. Methods: The seven cell subsets were isolated from spleen of tumor-bearing mice, embryonic liver (E14.5) and bone marrow of wild type mice by cell sorter respectively. Cells with purity >98% were used for subsequent experiments. The cell subsets RNA profiles were generated by deep sequencing,using Illumina. Results: Genes with FPKM values above 10.0 in the cell groups were used for subsequent analysis. Single linkage clustering analysis between the groups and prcomp function-based PCA analysis on the expression matrix were performed by R software. We found that the tumor-induced Ter-119+CD45−Lin− cells expressed the transcriptional factors and functional genes associated with erythrocyte and megakaryocyte development, but not myeloid cells differentiation.

2018-06-01 | GSE109429 | GEO

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