Project description:Transcriptome sequencing of naupliar- cyprid- and adult-specific normalised ESTs libraries of the acorn barnacle Balanus amphitrite.
Project description:The base of the barnacle Amphibalanus amphitrite has a layer of protein and cuticle that adheres the barnacle to the surface. The proteins in the adhesive have been characterized, but it is unknown whether the adhesive proteins are associated with the cuticle. To address this possibility, we examined the proteins associated with the molt (exuviae) from the main body, which is also composed of cuticle.
Project description:Settlement-inducing protein complex (SIPC) is a protein that acts as a phoromone cue to attract conspecific of the barnacle Amphibalanus amphitrite to settle on a substrate. Recombinant settlement-inducing protein complex was expressed in insect Sf9 cells through a baculovirus overexpression system, purified and analysed by mass spectrometry to confirm its identity.
