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RNA-seq analysis reveals profound changes in transcript profiles between siCon- and siH19-transfected uterine smooth muscle cells (USMC)

ABSTRACT: RNA-seq analysis reveals profound changes in transcript profiles between siCon- and siH19-transfected uterine smooth muscle cells (USMC)

PROVIDER: PRJNA433983 | ENA |

REPOSITORIES: ENA

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RNA-seq analysis reveals profound changes in transcript profiles between siCon- and siH19-transfected uterine smooth muscle cells (USMC)

Project description:We used high throughput sequencing to analyze the gene expression profiling of human uterine smooth muscle cells (USMC). By comparing the gene expression profiling of USMC with or without H19 knockdown, 215 genes were found at least 1.5-fold up-regulated in siH19 relative to siCon cells, meanwhile 228 genes were found at least 1.5-fold down-regulated in siH19 over siCon cells.

2019-10-01 | GSE110557 | GEO

RNA-seq analysis reveals profound changes in transcript profiles between siCon and siH19 transfected aortic smooth muscle cells (AoSMC)

Project description:We used high throughput sequencing to analyze the gene expression profiling of human aortic smooth muscle cells (AoSMC). By comparing the gene expression profiling of AoSMC with or without H19 knockdown, 2940 genes were found at least 1.5-fold up-regulated in siH19 relative to siCon cells, meanwhile 2790 genes were found at least 1.5-fold down-regulated in siH19 over siCon cells.

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RNA-seq analysis reveals profound changes in transcript profiles between siCon and siH19 transfected aortic smooth muscle cells (AoSMC)

Project description:RNA-seq analysis reveals profound changes in transcript profiles between siCon and siH19 transfected aortic smooth muscle cells (AoSMC)

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RNA-seq analysis reveals profound changes in transcript profiles between siCon- and siH19-transfected myotubes

Project description:We used high throughput sequencing to analyze the transcriptional profiling of myotubes. By comparing the transcriptional profiling of myotubes with or without H19 knockdown,1131 genes or transcripts were found at least 1.5-fold up-regulated in siH19 relative to siCon cells, meanwhile 508 genes or transcripts were found at least 1.5-fold down-regulated in siH19 over siCon cells. 40h following initiation of differentiation, myotubes were transfected with either control siRNA or siH19. 48h later after transfection, total RNA was extracted for library preparation and RNA-seq analysis to compare trancript profiles between siCon and siH19 cells.

2015-11-09 | E-GEOD-72941 | biostudies-arrayexpress

RNA-seq analysis reveals profound changes in transcript profiles between siCon- and siH19-transfected myotubes

2015-11-09 | GSE72941 | GEO

Transcription profiling of human Gene Expression in Uterine Smooth Muscle Tumors (including normal myometrium, benign uterine leiomyoma, malignant uterine and extra-uterine leiomyosarcoma)

Project description:Comparison of Gene Expression in Uterine Smooth Muscle Tumors (including normal myometrium, benign uterine leiomyoma, malignant uterine and extra-uterine leiomyosarcoma). Total RNA was isolated with Trizol and converted to labeled cRNA by standard Affymetrix protocols. Before analysis, Avg Diff values were normalized to a sum of 3 million for each probe set on the chip and values less than 20 were adjusted to 20 to allow log scaling in subsequent analysis.

2007-09-25 | E-GEOD-764 | biostudies-arrayexpress

Comparison of Gene Expression in Uterine Smooth Muscle Tumors

Project description:Comparison of Gene Expression in Uterine Smooth Muscle Tumors (including normal myometrium, benign uterine leiomyoma, malignant uterine and extra-uterine leiomyosarcoma. Total RNA was isolated with Trizol and converted to labeled cRNA by standard Affymetrix protocols. Before analysis, Avg Diff values were normalized to a sum of 3 million for each probe set on the chip and values less than 20 were adjusted to 20 to allow log scaling in subsequent analysis. Keywords: other

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Transcriptomic effects of estradiol treatment on cultured human uterine smooth muscle cells

Project description:The myometrium is an important reproductive tissue composed primarily of smooth muscle cells. Contractility of the myometrial smooth muscle cells during the estrous cycle and pregnancy is modulated by estrogen. Despite much research, little is known about the molecular mechanism by which estrogen regulates myometrial contractility. This study investigates global gene expression profile of cultured human uterine smooth muscle cells (hUtSMCs) following 17M-NM-2-estradiol (E2) treatment using cDNA microarray technology. In response to E2 treatment 540 genes were identified as significantly differentially expressed. Gene ontology analysis identified several significant biological processes for these genes including muscle contraction, gland development, cell migration, cell adhesion, apoptosis, response to external stimulus and phosphorylation. In this study evidence is presented that in human smooth muscle cells 17M-NM-2-estradiol, contrary to expectations, altered expression of several genes that may favor myometrial relaxation. Genes related to focal adhesion function were downregulated suggesting a loosening of SMC connections to the ECM while upregulation of MMP9 is also associated with weakened ECM interactions. The hUtSMC system is an additional useful model to investigate steroid effects on smooth muscle cells in isolation from other myometrial cell types. In this study human myometrial smooth muscle cells (hUtSMC) purchased from Lonza were cultured and treated with 17beta estradiol. RNA was isolated after 6h, 24h and 72h from both treated and untreated control cells. The experiment was repeated at least 3 times.

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Transcriptomic effects of estradiol treatment on cultured human uterine smooth muscle cells

Project description:The myometrium is an important reproductive tissue composed primarily of smooth muscle cells. Contractility of the myometrial smooth muscle cells during the estrous cycle and pregnancy is modulated by estrogen. Despite much research, little is known about the molecular mechanism by which estrogen regulates myometrial contractility. This study investigates global gene expression profile of cultured human uterine smooth muscle cells (hUtSMCs) following 17β-estradiol (E2) treatment using cDNA microarray technology. In response to E2 treatment 540 genes were identified as significantly differentially expressed. Gene ontology analysis identified several significant biological processes for these genes including muscle contraction, gland development, cell migration, cell adhesion, apoptosis, response to external stimulus and phosphorylation. In this study evidence is presented that in human smooth muscle cells 17β-estradiol, contrary to expectations, altered expression of several genes that may favor myometrial relaxation. Genes related to focal adhesion function were downregulated suggesting a loosening of SMC connections to the ECM while upregulation of MMP9 is also associated with weakened ECM interactions. The hUtSMC system is an additional useful model to investigate steroid effects on smooth muscle cells in isolation from other myometrial cell types.

2014-06-02 | GSE51470 | GEO

Characterization of Plexiform Leiomyomata Provide Further Evidence for Genetic Heterogeneity Underlying Uterine Fibroids

Project description:Plexiform leiomyomata are a histologically defined subgroup of benign uterine smooth muscle tumors based on their epitheliod cytology and abundant extracellular matrix. We used microarrays to compare plexiform leiomyomata to normal myometrium (smooth muscle of the uterine wall), typical leiomyomata, cellular or atypical leiomyomata and malignant leiomyosarcoma of the uterus. Keywords: tumor analysis

2008-05-01 | GSE9511 | GEO

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