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ChIP-chip from Escherichia coli CC72 cells with RNAP β’ subunit before and after osmotic stress.

ABSTRACT: ChIP-chip from Escherichia coli CC72 cells with RNAP β’ subunit before and after osmotic stress.

PROVIDER: PRJNA434714 | ENA |

REPOSITORIES: ENA

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ChIP-chip from Escherichia coli CC72 cells with RNAP β’ subunit before and after osmotic stress.

Project description:RNA polymerase (RNAP) is the key transcription machinery and its interaction with genomic DNA orchestrates gene expression in response to environmental cues. Dynamic interaction and localization between RNAP and nucleoid were observed before and after osmotic stress. Chromatin immunoprecipitation (ChIP) of RNAP β’ subunit together with chromatin profiling by ChIP-on-chip analysis demonstrated the dynamics of genome-wide RNAP-DNA interactions during osmotic stress.

2018-04-11 | GSE110831 | GEO

ChIP-chip of MG1655 with antibody against E. coli RNAP beta subunit under various conditions

2009-11-04 | GSE15588 | GEO

ChIP-chip of MG1655 with antibody against E. coli RNAP beta subunit under various conditions

Project description:We integrated RNAP binding regions (RBRs) and mRNA transcript abundance to determine segments of contiguous transcription originating from promoter regions. To measure RBRs at a genome scale, we employed a ChIP-chip method to E. coli K-12 MG1655 grown in the presence or absence of rifampicin under multiple growth conditions using antibody against E. coli RNAP beta subunit. A twelve ChIP-chip study using immunoprecipitated DNA (IP-DNA) from four separate culture conditions with and/or without rifampicin treatment. The high-density oligonucleotide tiling arrays used were consisted of 371,034 oligonucleotide probes spaced 25 bp apart (25-bp overlap between two probes) across the E. coli genome (NimbleGen). Experiments were conducted as biological duplicates or triplicates (different cultures).

2009-11-03 | E-GEOD-15588 | biostudies-arrayexpress

ChIP-Seq of RNAP in E. coli DY330

Project description:Distribution of RNAP beta' subunit (RpoC) on genomic sites was studied for exponentially growing Escherichia coli using ChIP-Seq.

2022-01-01 | GSE182850 | GEO

ChIP-chip of Sigma70 and RNAP Beta in vivo in E. coli MG1655

Project description:These data were used to infer the genome-wide localization of sigma70 and core RNAP beta subunit for the study "The transition between transcriptional initiation and elongation in E. coli is highly variable and often rate-limiting" (Reppas et al. 2006). This study analyzes transfrags with respect to the ChIP profile of the sigma70 and the beta subunit of RNA polymerase. Keywords: ChIP-chip; tiled analysis of mRNA expression

2006-10-19 | GSE6061 | GEO

ChAP-chip analysis of RpoA binding profiles on the B. subtilis chromosome in the intact and the C-terminal truncated RNAP alpha subunit expressing cells.

Project description:We constructed the B. subtilis strains in which the expression of the intact and the C-terminal truncated RNAP alpha subunit were induced by different stimulus, IPTG and xylose, under the control of Pspac and Pxyl promoters. Then, we performed ChAP-chip analysis to analyze the impact of alpha-CTD defect on genome-wide transcriptome profile.

2015-06-25 | E-MTAB-3385 | biostudies-arrayexpress

ChAP-chip analysis of RpoC binding profiles on the B. subtilis chromosome in the intact and the C-terminal truncated RNAP alpha subunit expressing cells.

Project description:We constructed the B. subtilis strains in which the expression of the intact and the C-terminal truncated RNAP alpha subunit were induced by different stimulus, IPTG and xylose, under the control of Pspac and Pxyl promoters. Then, we performed ChAP-chip analysis to analyze the impact of M-^UM-^AM-A-CTD defect on genome-wide transcriptome profile.

2015-06-25 | E-MTAB-3384 | biostudies-arrayexpress

ChIP-Seq of RNAP in E. coli DY330

Project description:ChIP-Seq of RNAP in E. coli DY330

| PRJNA758062 | ENA

ChIP-chip of RNA Polymerase (RNAP) and RpoD in Salmonella enterica serovar Typhimurium strain SL1344

Project description:ChIP-on-chip analysis of RNAP and RpoD binding to the Salmonella enterica serovar Typhimurium chromosome demonstrated a high degree of overlap between RNAP and RpoD binding and provided us with important insights into the global distribution of these factors. Furthermore this data was correlated with information on the location of 1873 transcription start sites identified by RNA-Seq technology, thereby providing a detailed transcriptional map of Salmonella Typhimurium. Analysis of RNAP, RNAP-Rifampicin and and RpoD binding in Luria Broth (LB)

2012-02-16 | E-GEOD-35827 | biostudies-arrayexpress

ChIp-Chip using RNAP II, CREB C/EBPb and cJun antibody in undifferentiated or differentiated keratinocytes (ChIP-Chip)

Project description:Combinatorial recruitment of CREB, C/EBPb and Jun determines activation of promoters upon keratinocyte differentiation Chromatin immunoprecipitation (ChIP) of RNAP II, CREB C/EBPb and cJun in undifferentiated or differentiated keratinocytes demonstrate recruitment of RNAP II to promoters bound by combination of specific transcription factors

2013-07-01 | GSE48381 | GEO

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