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Small RNA profiling of WT E14, Dgcr8_KO, Drosha_KO, Dicer_KO and Ago1&2_Double_KO mouse embryonic stem cells

ABSTRACT: Small RNA profiling of WT E14, Dgcr8_KO, Drosha_KO, Dicer_KO and Ago1&2_Double_KO mouse embryonic stem cells

PROVIDER: PRJNA435100 | ENA |

REPOSITORIES: ENA

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Small RNA profiling of WT E14, Ago2_KO and Ago2_KO-AGO1 mouse embryonic stem cells.

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small RNA Seq of Subcutaneous adipose tissue from endothelial-AGO1-knockout (EC-AGO1-KO) mice and wild-type (WT) littermates fed 16 week of high fat high sucrose diet

Project description:Purpose:to reveal the changes in small RNA, esp. microRNA to correlate with transcriptome changes occuring due to EC-AGO1-KO and further explain the observed phenotype. Methods: Subcutaneous adipose tissue were harvested.Small RNA library was prepared and for sequencing. Results: We found substantial changes in miRNA due to EC-AGO1-KO. Conclusions: Our study represents the detailed analysis of Ago1 regulated small RNA transcriptomes in subcutaneous adipose tissue between WT and KO mice. We conclude that small RNA-seq based transcriptome characterization would expedite genetic network analyses and permit the dissection of complex biologic functions.

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Project description:Endothelial-AGO1-knockout (EC-AGO1-KO) mice and wild-type (WT) littermates

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