Poa annua
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA435446 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The adult mammalian brain is composed of distinct regions that have specialized roles. The BF/POA regions are thought to have an important role in the regulation of sleep/wake behavior. However, genetic markers of the responsible cells for the regulation of sleep/wake behavior are largely unknown. To identify the molecular markers of the BF/POA regions, we sampled the BF/POA regions and compared gene expression in the BF/POA regions with those of other brain regions which we previously reported in the BrainStars (B*) project, in which we sampled ~50 small brain regions, including sensory centers and centers for motion, time, memory, fear, and feeding. We sampled each region every 4 hours for 24 hours, and pooled the sample sets for DNA-microarray assays. We then used informatics to identify candidates for genes with high or low expression in the BF/POA regions. We used our findings to develop an integrated database (http://poabf.brainstars.org/) for exploring genome-wide expression in the adult mouse brain including the BF/POA regions.
2018-08-17 | GSE107500 | GEO
Project description:Purpose: UV-B radiation is a pivotal photomorphogenic signal and positively regulates plant growth and metabolite biosynthesis. In order to elucidate the transcriptional regulation mechanism underlying UV-B-induced artemisinin and flavonoid biosynthesis in Artemisia annua, the transcriptional response of A. annua leaves to UV-B radiation was analyzed using the Illumina transcriptome sequencing. Methods: For UV-B treatment, six-week-old A. annua seedlings growing under normal growth condition were supplemented with extra narrowband UV-B lamps (Philips TL20W/01RS; 1.5 μmol·m-2·s-1)(Yin et al. 2016). The most recently expanded leaf for each A. annua seedling was collected at 0, 2, 4 and 6 hours after exposure to UV-B radiation. Results: A total of 10706 differentially expressed genes including 533 transcription factors, were identified. Based on the expression trends of the differentially expressed factors as well as artemisinin and flavonoid biosynthesis genes, we speculated that transcription factors belonging to 6 clusters were most likely to be involved in the regulation of artemisinin and/or flavonoid biosynthesis. The regulatory relationship between transcription factors and artemisinin/flavonoid biosynthetic genes was further studied. Dual-LUC assays results showed that AaMYB6 is a positive regulator of AaLDOX, which belongs to flavonoid biosynthesis pathway. In addition, we identified a R2R3MYB transcription factor, AaMYB4 which positively mediated both artemisinin and flavonoid biosynthesis pathways by activating the expression of AaADS and AaDBR2 in artemisinin biosynthesis pathway and AaUFGT in flavonoid biosynthesis pathway. Conclusions: our findings provide fundamental knowledge for the further analysis of the parallel transcriptional regulation of artemisinin and flavonoid biosynthesis in A. annua L. under UV-B radiation.
2023-01-01 | GSE159604 | GEO
Project description:The proliferative niches in the subpallium generate a rich cellular variety fated for diverse telencephalic regions. The embryonic preoptic area (POA) represents one of these domains giving rise to the pool of cortical GABAergic interneurons and glial cells, in addition to striatal and residual POA cells. The migration from sites of origin within the subpallium to the distant targets like the cerebral cortex, accomplished by the adoption and maintenance of a particular migratory morphology, is a critical step during interneuron development, which seems to be regulated partially via DNA methylation-dependent gene expression. To identify genes that are altered by DNA methylation mediated by DNMT1 in POA-derived Hmx3-positive interneurons, we used an Hmx3-Cre/tdTomato/Dnmt1loxP mouse model and FAC-sorted the basal telencephalon at E16. RNA and MeDIP sequencing were performed. MeDIP data are assigned here.
2021-03-09 | GSE146967 | GEO
Project description:The proliferative niches in the subpallium generate a rich cellular variety fated for diverse telencephalic regions. The embryonic preoptic area (POA) represents one of these domains giving rise to the pool of cortical GABAergic interneurons and glial cells, in addition to striatal and residual POA cells. The migration from sites of origin within the subpallium to the distant targets like the cerebral cortex, accomplished by the adoption and maintenance of a particular migratory morphology, is a critical step during interneuron development, which seems to be regulated partially via DNA methylation-dependent gene expression. To identify genes that are altered by DNA methylation mediated by DNMT1 in POA-derived Hmx3-positive interneurons, we used an Hmx3-Cre/tdTomato/Dnmt1loxP mouse model and FAC-sorted the basal telencephalon at E16. RNA and MeDIP sequencing were performed. RNA data are assigned here.
2021-03-09 | GSE146966 | GEO
Project description:Artemisia annua is known to produce the antimalarial phytomolecule artemisinin. The seedling and mature leaf of the plant represent two contrasting tissues in terms of their artemisinin content. The major objective of the present study was to use a small-scale (750 target genes) microarray of A. annua for identification of genes that are differentially expressed in the seedling and mature leaf tissues of the plant.
2014-12-31 | GSE39098 | GEO