Project description:Three mouse models of human central nervous system pathologies were selected for analyzing gene expression changes compared to wild type normal brain. The selected models are: TgAPP23 for Alzheimerï¾s disease (see Sturchler-Pierrat, C., Abramowski, D., Duke, M., Wiederhold, K.H., Mistl, C., Rothacher, S., Ledermann, B., B?rki, K., Frey, P., Paganetti, P.A., Waridel, C., Calhoun, M.E., Jucker, M., Probst, A., Staufenbiel, M. and Sommer, B. (1997) Two amyloid precursor protein transgenic mouse models with Alzheimer disease-like pathology. PNAS 94, 13287-13292), Tg6074 for multiple sclerosis (see Akassoglou K, Bauer J, Kassiotis G, Pasparakis M, Lassmann H, Kollias G and Probert L. (1998). Oligodendrocyte apoptosis and primary demyelination induced by local TNF/p55TNF receptor signaling in the central nervous system of transgenic mice: models for multiple sclerosis with primary oligodendrogliopathy. Am J Pathol. 153, 801-813) and permanent mid-cerebral artery occlusion for stroke (see Welsh, F.A., Sakamoto, T., McKee, A.E. and Sims, R.E. (1987) Effect of lactacidosis on pyridine nucleotide stability during ischemia in mouse brain. J Neurochem. 49, 846-851). Trangenic mice were grown to appropriate age, so that they would manifest the symptoms of disease or disease was induced by a surgical procedure to adult mice. Mice were sacrificed and half brains were removed at selected time points after disease onset for RNA extraction and subsequent array hybridization.
2008-07-12 | E-MTAB-1 | biostudies-arrayexpress
