Project description:In order to reveal so far unknown facets of the adaptation of B. subtilis to growth under high-salinity conditions, a whole-transcriptome analysis of B. subtilis BSB (168 Trp+) was performed using strand-specific tiling arrays (tiling step of 22 nucleotides). In addition, the effects of glycine betaine (GB) were analyzed under high salinity and standard growth conditions in a chemically defined medium. Important novel findings were a sustained low-level induction of the SigB-dependent general stress response and strong repression of biofilm matrix genes under high-salinity conditions. GB influences gene expression not only under high-salinity, but also under standard growth conditions without additional salt.
Project description:Identification of key steps and associated microbial populations for efficient anaerobic digestion under high ammonium and salinity conditions
Project description:LCMS of mangrove propagule extracts incubated under different conditions (high vs low salinity, antibiotic vs antibiotic+antifungal vs control).
