Project description:Investigation of whole genome gene expression level changes in Ferroglobus placidus grown on phenol versus acetate as electron donors, with ferric citrate as electron acceptor.
Project description:Investigation of whole genome gene expression level changes in Ferroglobus placidus grown on phenol versus acetate as electron donors, with ferric citrate as electron acceptor. A six chip study using total RNA recovered from three separate cultures of Ferroglobus placidus DSM 10642 grown with 0.5 mM phenol and three separate cultures of Ferroglobus placidus DSM 10642 grown on 10 mM acetate. Each chip measures the expression level of 2613 genes from Ferroglobus placidus DSM 10642 with nine 45-60-mer probe pairs (PM/MM) per gene, with three-fold technical redundancy.
Project description:This SuperSeries is composed of the following subset Series: GSE28549: Anaerobic Oxidation of Benzene by the Hyperthermophilic Archaeon Ferroglobus placidus (Phenol vs. Benzoate) GSE30798: Anaerobic Oxidation of Benzene by the Hyperthermophilic Archaeon Ferroglobus placidus (Benzene vs. Acetate) GSE30799: Anaerobic Oxidation of Benzene by the Hyperthermophilic Archaeon Ferroglobus placidus (Benzene vs. Phenol) GSE30801: Anaerobic Oxidation of Benzene by the Hyperthermophilic Archaeon Ferroglobus placidus (Benzene vs. Benzoate) Refer to individual Series
Project description:Comparison of acetate- to phenylacetate-grown F. placidus cells to identify genes that are potentially involved in anaerobic phenylacetate degradation by this unique hypertherophilic archaeon. A four chip study using total RNA recovered from two separate cultures of Ferroglobus placidus DSM 10642 grown with 1 mM phenylacetate (experimental condition) and two separate cultures of Ferroglobus placidus DSM 10642 grown on 10 mM acetate (control condition). Each chip measures the expression level of 2613 genes from Ferroglobus placidus DSM 10642 with nine 45-60-mer probe pairs (PM/MM) per gene, with three-fold technical redundancy.
Project description:Comparison of acetate- to butyrate-grown F. placidus cells to identify genes that are potentially involved in fatty acid degradation by this unique hypertherophilic archaeon. A four chip study using total RNA recovered from three separate cultures of Ferroglobus placidus DSM 10642 grown with 1 mM butyrate (experimental condition) and three separate cultures of Ferroglobus placidus DSM 10642 grown on 10 mM acetate (control condition). Each chip measures the expression level of 2613 genes from Ferroglobus placidus DSM 10642 with nine 45-60-mer probe pairs (PM/MM) per gene, with three-fold technical redundancy.