Project description:We indirectly cocultured OVCAR5-RFP cells using a 0.45 micron Boyden chamber with or without adipocytes for 4 days, collected the RNA from adipocytes, and performed RNA-seq
Project description:We performed RNA sequencing to compare the transcriptomes of 3T3-L1 mature adipocytes alone and cocultured with PC cell line Panc-1 cells for 5.
Project description:Analysis of changes on gene expression levels in differentiating adipocytes treated with differentiation medium (IBMX), insulin and dexamethasone (time points 2d-4d) as well as with maturation medium containing insulin (time points 6d - 18d)
Project description:This study characterized the genome-wide binding of histone 3 lysine 79 tri-methylation and expression profile of DOT1L knockdown in OVCAR5 cells.
Project description:This study characterized the genome-wide binding of histone 3 lysine 79 tri-methylation and expression profile of DOT1L knockdown in OVCAR5 cells.
Project description:This study characterized the genome-wide binding of FOXK2 in ovarian cancer (OC) cell OVCAR-5 and investigated the expression profile of OVCAR5 cells with FOXK2 knocking-down.
Project description:This study characterized the genome-wide binding of FOXK2 in ovarian cancer (OC) cell OVCAR-5 and investigated the expression profile of OVCAR5 cells with FOXK2 knocking-down.
Project description:We developed a CHK1 inhibitor Prexasertib resistant strain of ovarian cancer cell line OVCAR5 in lab. This was used to compare transcriptome profiles to study changes in expression profiles of genes that might be involved with resistance. Total RNA was isolated from three replicate cultures of OVCAR5R (IC50 viability >3 micromolar Prexasertib) and 3 replicate cultures of parental OVCAR5 (IC50 viability=7.5nM) and then subjected to Hiseq4000 illumina RNA-seq analysis, with paired end sequencing. Data file contains CPM-TMM values after zoom normalization.