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Effect of transgenic RNAi on wandering third instar larval fat body gene expression in Drosophila melanogaster

ABSTRACT: Effect of transgenic RNAi on wandering third instar larval fat body gene expression in Drosophila melanogaster

PROVIDER: PRJNA437974 | ENA |

REPOSITORIES: ENA

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Expression profile of third instar larval fat body and midgut tissues

Project description:We use mRNA-seq to transcriptionally profile larval fat body and midgut tissues from Drosophila third instar larvae. These data provide insights into tissue physiology and can be used to identify tissue specific transcripts. Fat bodies from wandering third instar larvae were dissected from ~50 male larvae and gonads were removed to eliminate contaminating transctips from the gonads. Larval midguts were dissected from ~50 wandering third instar larvae. Larval tissues were removed to Graces unsupplemented medium on ice prior to RNA extraction with TRIzol reagent. mRNA-seq samples were prepared from 5ug of total RNA and subject to Illumina based sequencing.

2010-11-02 | E-GEOD-25025 | biostudies-arrayexpress

Effect of EcR RNAi on wandering third instar larval fat body gene expression in Drosophila melanogaster

Project description:Effect of EcR RNAi on wandering third instar larval fat body gene expression in Drosophila melanogaster

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Expression profile of third instar larval salivary gland tissue

Project description:We use mRNA-seq to transcriptionally profile larval salivary gland tissue from Drosophila third instar larvae. These data provide insights into tissue physiology and can be used to identify tissue specific transcripts. Salivary glands were dissected from 200 wandering third instar larvae and the associated fat body was removed.Salivary glands were transferred to Graces unsupplemented medium on ice prior to RNA extraction with TRIzol reagent. mRNA-seq samples were prepared from 10 ug of total RNA and subject to Illumina based sequencing.

2011-10-18 | E-GEOD-33017 | biostudies-arrayexpress

Expression profile of third instar larval fat body and midgut tissues

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Effect of transgenic RNAi on wandering third instar larval fat body gene expression in Drosophila melanogaster

Project description:We compared four transcription factor knockdowns using transgenic RNAi expressed in the larval fat body. FOXO, Tfb1, p53, and Stat92E-dependent gene expression in the Drosophila fat body was quantified on control and high-sugar diets in order to generate expression profiles via RNA-seq. These expression data were used to build a gene regulatory network to predict novel roles for these and other genes during caloric overload.

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Expression data from 0.15M and 0.7M-fed wild-type and ChREBP mutant, third instar Drosophila larval fat bodies (FBs)

Project description:Chronic high sugar feeding induces obesity, hyperglycemia, and insulin resistance in flies and mammals. These phenotypes are controlled by the fat body, a liver- and adipose- like tissue in Drosophila flies. To gain insight into the mechanisms underlying the connection between diet and insulin sensitivity, we used Illumina RNA-seq to profile gene expression in fat bodies isolated from chronically high sugar fed, wandering (post-prandial) third instar wild type larvae w(L3). These data were compared to control-fed wild-type wL3 fat bodies as well as those expressing transgenic interfering RNA (i) targeting CG18362 (Mio/dChREBP) in the fat body on both diets. Female VDRC w1118, cgGAL4, UAS-Dcr2 or UAS-ChREBPi(52606), cgGAL4, UAS-Dcr2 wandering third instar larvae were fed control (0.15M) or high (0.7M) sucrose and fat bodies isolated for RNA extraction.

2013-01-25 | E-GEOD-43734 | biostudies-arrayexpress

Expression profile of third instar larval salivary gland tissue

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ChIP-Seq of ORC2 bound to third instar salivary gland DNA in WT and mutant Drosophila, analyzed by Illumina sequencing

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Effect of EcR RNAi on wandering third instar larval fat body gene expression in Drosophila melanogaster

Project description:We compared ecdysone receptor (EcR)-dependent gene expression in the Drosophila fat body on 0.15 M sucrose and 0.5 M sucrose high-sugar diets in order to gain insight into the role of this gene during caloric overload. Phenotypic analyses showed an increased severity of EcR RNAi phenotypes with increasing dietary sugar concentration. Because EcR is a transcription factor, we performed RNA-seq studies to identify transcriptional targets that might underlie insulin resistance downstream of EcR RNAi.

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2013-02-27 | E-MTAB-699 | biostudies-arrayexpress

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