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Mnemiopsis leidyi high resolution developmental transcriptomic time-course 0-20 hours

ABSTRACT: Mnemiopsis leidyi high resolution developmental transcriptomic time-course 0-20 hours

PROVIDER: PRJNA438062 | ENA |

REPOSITORIES: ENA

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Mnemiopsis leidyi high resolution developmental transcriptomic time-course 0-20 hours

Project description:These data complement GEO accession GSE60478, a time-course of Mnemiopsis leidyi developmental gene expression for hours 0-13 and hour 20. The data here provide additional replicates for 0-13 and 20 hours as well as new timepoints 14–19. As in the original study (Levin et al. 2016 PMID: 26886793) data are from single embryos using the Cel-Seq (Single-Cell RNA-Seq by Multiplexed Linear Amplification PMID:22939981). Embryo processing, sequencing and sequence processing were performed in the same manner as the original analyses (GEO accession GSE60478) so these data should be completely compatible with that study.

2018-03-14 | GSE111748 | GEO

Mnemiopsis leidyi

Project description:Mnemiopsis leidyi

| PRJNA816735 | ENA

Mnemiopsis Embryonic Developmental Timecourse

Project description:A time series from 1 hour to 9 hours post-fertilization (hpf) was collected for RNA-seq by students of the MBL Embryology Course to understand the shifts in gene expression occuring in the early embryo of Mnemiopsis leidyi.

2018-01-23 | GSE93977 | GEO

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Project description:Mnemiopsis leidyi genome sequencing

| PRJNA280770 | ENA

Mnemiopsis leidyi

Project description:Mnemiopsis leidyi Raw sequence reads

| PRJNA689379 | ENA

Mnemiopsis leidyi

Project description:Mnemiopsis leidyi Transcriptome or Gene expression

| PRJNA344880 | ENA

Mnemiopsis leidyi

Project description:Mnemiopsis leidyi high resolution developmental transcriptomic time-course

| PRJNA258375 | ENA

Mnemiopsis leidyi

Project description:Regeneration in the Ctenophore Mnemiopsis leidyi: A Transcriptomic Analysis

| PRJNA305523 | ENA

Mnemiopsis leidyi

Project description:Single- and eight-cell stage Mnemiopsis leidyi RNAseq raw sequence reads

| PRJNA396768 | ENA

Mnemiopsis leidyi high resolution developmental transcriptomic time-course

Project description:Classical embryological studies revealed that during mid-embryogenesis vertebrates show similar morphologies. This “phylotypic stage” has recently received support from transcriptome analyses, which have also detected similar stages in nematodes and arthropods. A conserved stage in these three phyla has led us to ask if all animals pass through a universal definitive stage as a consequence of ancestral constraints on animal development. Previous work has suggested that HOX genes may comprise such a ‘zootypic’ stage, however this hypothetical stage has hitherto resisted systematic analysis. We have examined the embryonic development of ten different animals each of a fundamentally different phylum, including a segmented worm, a flatworm, a roundworm, a water bear, a fruitfly, a sea urchin, a zebrafish, a sea anemone, a sponge, and a comb jelly. For each species, we collected the embryonic transcriptomes at ~100 different developmental stages and analyzed their gene expression profiles. We found dynamic gene expression across all of the species that is structured in a stage like manner. Strikingly, we found that animal embryology contains two dominant modules of zygotic expression in terms of their protein domain composition: one involving proliferation, and a second involving differentiation. The switch between these two modules involves induction of the zootype; which in addition to homeobox containing genes, also involves Wnt and Notch signaling as well as forkhead domain transcription factors. Our results provide a systematic characterization of animal universality and identify the points of embryological constraints and flexibility.

2016-02-18 | GSE60478 | GEO

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